Endocytosis-mediated siderophore uptake as a strategy for Fe acquisition in diatoms.
Endocytosis-mediated siderophore uptake as a strategy for Fe acquisition in diatoms.
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DOI：
10.1126/sciadv.aar4536
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发表时间：
2018-05
期刊：
影响因子：
13.6
通讯作者：
Lesuisse E
中科院分区：
文献类型：
--
作者：
Kazamia E;Sutak R;Paz-Yepes J;Dorrell RG;Vieira FRJ;Mach J;Morrissey J;Leon S;Lam F;Pelletier E;Camadro JM;Bowler C;Lesuisse E
我们报告了一种在全球重要的单细胞光合生物中获取铁的新策略。在广阔的海洋区域，由于铁的生物利用度较低，浮游植物的生长受到限制。铁施肥通常会导致硅藻大量繁殖，但硅藻如何在长期缺铁的水域中生存并在铁可用时超越其他浮游植物的生理基础尚未解决。我们表明，一些硅藻可以使用铁载体结合的铁，并表现出对铁载体类型的物种特异性识别。在三角褐指藻中，异羟肟酸铁载体在没有事先还原的情况下通过高亲和力机制被摄取，该机制涉及与细胞表面结合，然后通过内吞作用介导的摄取并递送至叶绿体。所记录的亲和力是任何真核细胞中铁转运系统所描述的最高亲和力。总的来说，我们的观察表明，除了完善的还原机制之外，硅藻中可能还存在多种铁吸收机制。我们发现铁饥饿诱导蛋白 1 (ISIP1) 在铁载体的摄取中发挥重要作用，通过生物信息学分析，我们推断该蛋白很大程度上是硅藻特异性的。我们通过查询塔拉海洋真核基因图谱来量化全球海洋中 ISIP1 的表达，并显示硅藻相关 ISIP1 的丰度和分布与慢性铁饥饿定义的海洋省份之间的联系。
We report a new strategy uncovered for iron acquisition in globally important unicellular photosynthetic organisms. Phytoplankton growth is limited in vast oceanic regions by the low bioavailability of iron. Iron fertilization often results in diatom blooms, yet the physiological underpinnings for how diatoms survive in chronically iron-limited waters and outcompete other phytoplankton when iron becomes available are unresolved. We show that some diatoms can use siderophore-bound iron, and exhibit a species-specific recognition for siderophore types. In Phaeodactylum tricornutum, hydroxamate siderophores are taken up without previous reduction by a high-affinity mechanism that involves binding to the cell surface followed by endocytosis-mediated uptake and delivery to the chloroplast. The affinity recorded is the highest ever described for an iron transport system in any eukaryotic cell. Collectively, our observations suggest that there are likely a variety of iron uptake mechanisms in diatoms besides the well-established reductive mechanism. We show that iron starvation–induced protein 1 (ISIP1) plays an important role in the uptake of siderophores, and through bioinformatics analyses we deduce that this protein is largely diatom-specific. We quantify expression of ISIP1 in the global ocean by querying the Tara Oceans atlas of eukaryotic genes and show a link between the abundance and distribution of diatom-associated ISIP1 with ocean provinces defined by chronic iron starvation.
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DOI：
10.1139/b05-077
发表时间：
2005-07-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
Granum, E;Raven, JA;Leegood, RC
通讯作者：
Leegood, RC
影响因子：
7.7
作者：
Dorrell, Richard G.;Gile, Gillian;Bowler, Chris
通讯作者：
Bowler, Chris
影响因子：
2.9
作者：
Kustka, Adam B.;Allen, Andrew E.;Morel, Francois M. M.
通讯作者：
Morel, Francois M. M.
影响因子：
3
作者：
Falciatore, A;Casotti, R;Bowler, C
通讯作者：
Bowler, C
影响因子：
64.8
作者：
Hutchins, DA;Witter, AE;Luther, GW
通讯作者：
Luther, GW