The inhibitory effect of C-reactive protein on bacterial phosphorylcholine platelet-activating factor receptor-mediated adherence is blocked by surfactant
The inhibitory effect of C-reactive protein on bacterial phosphorylcholine platelet-activating factor receptor-mediated adherence is blocked by surfactant
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DOI：
10.1086/341658
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发表时间：
2002-08-01
影响因子：
6.4
通讯作者：
Weiser, JN
中科院分区：
文献类型：
--
作者：
Gould, JM;Weiser, JN
人类呼吸道的许多主要病原体，包括肺炎链球菌和流感嗜血杆菌，都表达细胞表面磷胆碱(CHOP)，这是血小板激活因子(RPAF)受体的配体。CHOP还与C-反应蛋白(CRP)结合，在补体存在的情况下，C-反应蛋白可能具有杀菌作用。这项研究发现，CRP可以阻止表达细胞表面CHOP的细菌与宿主细胞的附着。相当于人呼吸道粘膜表面的CRP浓度阻止了肺炎链球菌和流感嗜血杆菌对人咽上皮细胞的大部分黏附。在rPAF拮抗剂存在的情况下，CHOP介导的黏附也被减少。RPAF拮抗剂和CRP的抗黏附作用不是相加的，提示CRP的活性是受体介导的黏附区域所特有的。人表面活性物质(主要是CHOP)抑制了CRP与CHOP的结合以及CRP对黏附的影响。在表面活性物质丰富的终末气道，CRP的抗黏附作用可能会减弱。
Numerous major bacterial pathogens in the human respiratory tract, including Streptococcus pneumoniae and Haemophilus influenzae, express cell-surface phosphorylcholine (ChoP), a ligand for the receptor for platelet-activating factor (rPAF). ChoP is also bound by C-reactive protein (CRP), which, in the presence of complement, may be bactericidal. This study found that CRP can block the attachment of bacteria expressing cell-surface ChoP to host cells. Concentrations of CRP equivalent to those on the mucosal surface of the human airway blocked most adherence of both S. pneumoniae and H. influenzae to human pharyngeal epithelial cells. ChoP-mediated adherence was also reduced in the presence of an rPAFantagonist. The antiadhesive effects of the rPAF antagonist and CRP were not additive, suggesting that CRP activity is specific to the area of adherence mediated by the receptor. The binding of CRP to ChoP and the effect of CRP on adherence were inhibited by human surfactant (primarily ChoP). The antiadhesive effect of CRP may be diminished in the terminal airway, where surfactant is abundant.