Expression of SLAM (CD150) cell-surface receptors on human B-cell subsets: From pro-B to plasma cells
Expression of SLAM (CD150) cell-surface receptors on human B-cell subsets: From pro-B to plasma cells
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DOI：
10.1016/j.imlet.2010.09.021
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发表时间：
2011-01-30
影响因子：
4.4
通讯作者：
Engel, Pablo
中科院分区：
文献类型：
--
作者：
De Salort, Jose;Sintes, Jordi;Engel, Pablo
SLAM (CD150) 家族受体是参与白细胞激活的白细胞细胞表面糖蛋白。这些分子及其衔接蛋白 SAP 有助于有效的生发中心形成、高亲和力抗体分泌浆细胞和记忆 B 细胞的生成，从而促进长期体液免疫反应。进行多色流式细胞术分析以确定人细胞系上 CD48 (SLAMF2)、CD84 (SLAMF5)、CD150 (SLAM 或 SLAMF1)、CD229 (Ly9 或 SLAMF3)、CD244 (2B4 或 SLAMF4)、CD319 (CRACC、CS1 或 SLAMF7) 和 CD352 (NTB-A 或 SLAMF6) 的表达，以及B 细胞亚群。评估了以下亚群：来自骨髓的原 B 细胞、前 B 细胞、未成熟 B 细胞和成熟 B 细胞；来自外周血的过渡型和 B1/B2 亚型；幼稚细胞、前生发中心、生发中心、记忆细胞、浆母细胞以及来自扁桃体和脾脏的浆细胞。除 CD244 外，所有受体均在 B 细胞上表达。 SLAM家族分子在B细胞发育、成熟和终末分化为浆母细胞和浆细胞过程中广泛分布，但其在不同B细胞亚群中的表达存在显着差异。这种异质的表达模式表明 SIAM 分子在体液免疫反应的控制中发挥着重要且非冗余的作用。 (C) 2010 Elsevier B.V. 保留所有权利。
The SLAM (CD150) family receptors are leukocyte cell-surface glycoproteins involved in leukocyte activation. These molecules and their adaptor protein SAP contribute to the effective germinal center formation, generation of high-affinity antibody-secreting plasma cells, and memory B cells, thereby facilitating long-term humoral immune response. Multi-color flow cytometric analysis was performed to determine the expression of CD48 (SLAMF2), CD84 (SLAMF5), CD150 (SLAM or SLAMF1), CD229 (Ly9 or SLAMF3), CD244 (2B4 or SLAMF4), CD319 (CRACC, CS1, or SLAMF7), and CD352 (NTB-A or SLAMF6) on human cell lines and B-cell subsets. The following subsets were assessed: pro-B, pre-B, immature-B, and mature-B cells from bone marrow; transitional and B1/B2 subsets from peripheral blood; and naive, pre-germinal center, germinal center, memory, plasmablasts, and plasma cells from tonsil and spleen. All receptors were expressed on B cells, with the exception of CD244. SLAM family molecules were widely distributed during B-cell development, maturation and terminal differentiation into plasmablasts and plasma cells, but their expression among various B-cell subsets differed significantly. Such heterogeneous expression patterns suggest that SIAM molecules play an essential and non-redundant role in the control of humoral immune responses. (C) 2010 Elsevier B.V. All rights reserved.