REFLECTIVE PROPERTIES OF THE STIGMA IN MALE GAMETES OF ECTOCARPUS SILICULOSUS (PHAEOPHYCEAE) STUDIED BY CONFOCAL LASER SCANNING MICROSCOPY 1
REFLECTIVE PROPERTIES OF THE STIGMA IN MALE GAMETES OF ECTOCARPUS SILICULOSUS (PHAEOPHYCEAE) STUDIED BY CONFOCAL LASER SCANNING MICROSCOPY 1
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共焦激光扫描显微镜研究黄藻雄配子柱头的反射特性1
DOI：
10.1111/j.0022-3646.1991.00268.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
M. Melkonian
中科院分区：
文献类型：
--
作者：
G. Kreimer;H. Kawai;D. Müller;M. Melkonian
本文研究了硅叶香豆（Ectocarpus siliculosus（Dillw.）使用共聚焦激光扫描显微镜在落射对比模式下研究Lyngbye。光学XY（水平）和XZ（垂直）切片后获得的复杂的反射模式是一致的柱头超微结构所揭示的连续薄切片。反射信号的强度和图案随着细胞/柱头对入射激光的取向而变化。最大反射仅发生在柱头对光源的大致正常方向上。聚焦的反射光从一个细长的凹凹陷的柱头上的鞭毛肿胀的区域观察到在XY和XZ部分的生活和固定的配子。结果表明，除了四分之一波干涉反射之外，其他机制（聚焦）在鞭毛虫藻类眼点的信号放大中的重要性。
The reflection properties of the stigma in male gametes of Ectocarpus siliculosus (Dillw.) Lyngbye were investigated using confocal laser scanning microscopy in the epireflection contrast mode. The complex reflection pattern obtained after optical xy (horizontal) and xz (vertical) sectioning was consistent with stigma ultrastructure as revealed by serial thin sections. The intensity and pattern of the reflection signal varied with the orientation of the cell/stigma to the incident laser light. Maximal reflection occurred only in approximately normal orientation of the stigma to the light source. Focusing of reflected light from an elongated concave depression of the stigma on the region of the flagellar swelling was observed in xy and xz sections of living and fixed gametes. The results indicate the importance of mechanisms (focusing) other than quarter‐wave interference reflection in signal amplification by the eyespot of flagellate algae.