Murine bladder wall biomechanics following partial bladder obstruction
Murine bladder wall biomechanics following partial bladder obstruction
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DOI：
10.1016/j.jbiomech.2013.07.022
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发表时间：
2013-10-18
影响因子：
2.4
通讯作者：
Pope, John C.
中科院分区：
文献类型：
--
作者：
Chen, Joseph;Drzewiecki, Beth A.;Pope, John C.
膀胱壁力学行为的评估对于理解在病理过程（如部分膀胱出口梗阻（pBOO））中发生的功能变化是重要的。在小鼠模型中，由于膀胱容量小和手术暴露膀胱，传统的膀胱测量方法难以描述膀胱顺应性。在这里，我们探索了一种替代技术，通过对经历pBOO的小鼠膀胱施加双轴机械拉伸来表征小鼠的力学特性。5-6周龄雌性C57/BI6小鼠切除卵巢，经开放手术尿道结扎行pBOO， 4周后处死（n=12）。年龄匹配的对照组（n=6）也进行了分析。在采集时根据纤维化（n=6）或膨胀（n=6）的表型分离膀胱。在改良的Kreb's溶液中进行双轴试验，温度为37℃。组织预处理10次，通过比较50 kPa下的轴向应变来评估力学响应。正常小鼠膀胱呈各向异性，纵向较硬。所有小鼠在pBOO治疗4周后均表现出各向异性的丧失。pBOO后观察到的两种表型，纤维化和膨胀，分别表现出更少和更多的可扩展性。这些证明原理的数据表明，pBOO在小鼠膀胱力学中产生可量化的变化，可以通过双轴测试有效地量化。(C) 2013年Elsevier Ltd.出版
Evaluation of bladder wall mechanical behavior is important in understanding the functional changes that occur in response to pathologic processes such as partial bladder outlet obstruction (pBOO). In the murine model, the traditional approach of cystometry to describe bladder compliance can prove difficult secondary to small bladder capacity and surgical exposure of the bladder. Here, we explore an alternative technique to characterize murine mechanical properties by applying biaxial mechanical stretch to murine bladders that had undergone pBOO.5-6 week old female C57/BI6 mice were ovariectomized and subjected to pBOO via an open surgical urethral ligation and sacrificed after 4 weeks (n=12). Age matched controls (n=6) were also analyzed. Bladders were separated based on phenotype of fibrotic (n=6) or distended (n=6) at the time of harvest. Biaxial testing was performed in modified Kreb's solution at 37 degrees C Tissue was preconditioned to 10 cycles and mechanical response was evaluated by comparing axial strain at 50 kPa. The normal murine bladders exhibited anisotropy and were stiffer in the longitudinal direction. All mice showed a loss of anisotropy after 4 weeks of pBOO. The two phenotypes observed after pBOO, fibrotic and distended, exhibited less and more extensibility, respectively. These proof-of-principle data demonstrate that pBOO creates quantifiable changes in the mechanics of the murine bladder that can be effectively quantified with biaxial testing. (C) 2013 Published by Elsevier Ltd.