Easy and accessible way to calibrate a fluorescence microscope and to create a microplastic identification key.
Easy and accessible way to calibrate a fluorescence microscope and to create a microplastic identification key.
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DOI：
10.1016/j.mex.2023.102053
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发表时间：
2023
期刊：
影响因子：
1.9
通讯作者：
Lynch, Iseult
中科院分区：
文献类型：
--
作者：
Kukkola, Anna;Krause, Stefan;Yonan, Yasmin;Kelleher, Liam;Schneidewind, Uwe;Smith, Gregory H. Sambrook;Nel, Holly;Lynch, Iseult
关键词：
微塑料鉴定键的开发和测试，以减少假阳性检测。使用检测限和鉴定键获得的微塑料鉴定的主观性较低。我们在这里提出了一种技术，可以在任何荧光显微镜上设置检测限，并结合荧光团尼罗红进行微塑料鉴定。我们的方法还描述了一个严格的微塑料形态特异性识别关键，以减少研究人员之间的主观性。建立了九种常见聚合物类型和五种天然底物的检出限，这些底物在使用尼罗河红进行微塑料鉴定时可能导致假阳性信号。然后将该方法应用于实际淡水样品，并通过微傅里叶红外或拉曼光谱对鉴定的颗粒进行了验证。这种方法可以减少微塑料识别和计数的主观性，提高微塑料研究界的透明度、可重复性和协调性。
Instructions for calibration of detection limits for microplastics on fluorescence microscope systems described Microplastic identification key developed and tested to reduce false positive detection Lower subjectivity for microplastic identification obtained using the detection limits & identification key We present here a technique for setting up detection limits on any fluorescent microscope in conjunction with the fluorophore Nile Red for microplastic identification. Our method also describes a rigorous morphology-specific identification key for microplastics to reduce subjectivity between researchers. The detection limits were established for nine common polymer types and five natural substrates which could result in false-positive signals when using Nile Red for microplastic identification. This method was then applied to real freshwater samples and identified particles were validated with micro-FTIR or Raman spectroscopy. This approach may reduce subjectivity in microplastic identification and counting and enhances transparency, repeatability and harmonization within microplastic research community.
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