Growth enhancement of Bifidobacterium lactis Bo and Lactobacillus acidophilus Ki by milk hydrolyzates
Growth enhancement of Bifidobacterium lactis Bo and Lactobacillus acidophilus Ki by milk hydrolyzates
复制标题
DOI：
10.3168/jds.s0022-0302(98)75840-0
复制
发表时间：
1998-11-01
影响因子：
3.5
通讯作者：
Klaver, FAM
中科院分区：
文献类型：
--
作者：
Gomes, AMP;Malcata, FX;Klaver, FAM
如果要实现一致可靠的酸生产，则确定可能适合于仅由乳双歧杆菌Bo和嗜酸乳杆菌Ki组成的奶酪制作的（暂定）益生菌起子培养物的最佳制备条件是至关重要的，特别是因为双歧杆菌对生长具有严格的要求。因此，我们确定是否B。lactis Bo和L.嗜酸乳杆菌Ki需要或受益于添加乳水解产物（由蛋白酶或中性蛋白酶作为氮源产生）。B的生长产酸量。牛奶中的乳酸受到蛋白酶介导的水解产物的影响，并在较小程度上，由中性蛋白酶介导的水解产物;水解产物的水解程度越高，导致更大的生物量增加和更大的产酸量。这一结果表明，双歧杆菌在牛奶中生长不良部分是由于缺乏小肽和游离氨基酸。B的生长速率和酸化速率。乳酸菌与L.嗜酸乳杆菌（1：1接种比例）。相反，L.嗜酸乳杆菌没有受到任何牛奶水解物的添加的积极影响。虽然L.嗜酸乳杆菌生长缓慢，其蛋白水解系统显然能够产生自己的氮源。然而，与B共培养。乳酸菌（1：1接种比例）导致增强的增长和酸化速率时，与单一菌株相比，这表明一定程度的菌株之间的共生。
The determination of the best conditions of preparation of a (tentatively) probiotic starter culture that might be suitable for cheese making composed solely of Bifidobacterium lactis Bo and Lactobacillus acidophilus Ki is critical if a consistently reliable acid production is to be achieved, especially because bifidobacteria have stringent requirements for growth. Therefore, we determined whether B. lactis Bo and L. acidophilus Ki required or benefitted from the addition of milk hydrolyzates (brought about by proteinase or neutrase as the nitrogen source). The growth acid acid production of B. Lactis in milk were affected by the addition of proteinase-mediated hydrolyzate and, to a lesser extent, by neutrase-mediated hydrolyzate; a higher degree of hydrolysis of either hydrolyzate resulted in greater biomass increase and greater acid production. This result suggests that the poor growth of bifidobacteria in milk is due partially to the lack of small peptides and free amino acids. The rates of growth and acidification by B. lactis were enhanced when cocultured with L. acidophilus (1:1 inoculum ratio). Conversely, the growth rates and acid production of L. acidophilus were not positively affected by the addition of either milk hydrolyzate. Although L. acidophilus grew slowly, its proteolytic system was apparently able to generate its own nitrogen source. Nevertheless, coculture with B. lactis (1:1 inoculum ratio) led to enhanced rates of growth and acidification when compared with that of the single strain, suggesting some degree of symbiosis between the strains.