The stimulation of myoblast differentiation by electrically conductive sub-micron fibers
The stimulation of myoblast differentiation by electrically conductive sub-micron fibers
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DOI：
10.1016/j.biomaterials.2008.12.063
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发表时间：
2009-04-01
期刊：
影响因子：
14
通讯作者：
Shin, Heungsoo
中科院分区：
文献类型：
--
作者：
Jun, Indong;Jeong, Sungin;Shin, Heungsoo
肌管与一束束的肌纤维组装在一起，形成骨骼肌的结构单元。因此，肌管的形成在恢复肌肉功能方面起着重要的作用，而促进成肌细胞向肌管分化的底物需要为肌肉组织工程开发。本研究采用静电纺丝法制备了聚(L-丙交酯-己内酯-己内酯)与聚苯胺(PANI)共混的导电复合纤维，并研究了其对成肌细胞分化的影响。无论PANI的加入与否，制备的PlcL/PANI纤维的直径和接触角均无显著差异。含30%PANI(PlcL/PANI-30)的纤维保持了最大断裂伸长率(160+/-14.4%)的弹性性能。在C2C12成肌细胞培养的8天内，每根纤维上的DNA含量相似，因此复合纤维具有细胞相容性。培养4天后，肌球蛋白阳性细胞数在导电纤维上(PlcL/PANI-15和-30纤维分别为21+/-1和19+/-2)是PlcL/PANI-0纤维(6+/-2)的3.6倍。此外，在培养第8天，在PlcL/PANI-15纤维上检测到的肌生成素表达水平大约是PlcL/PANI-0纤维上的1.6倍。其他基因的表达也有类似的结果，包括肌钙蛋白T(增加2倍)和肌球蛋白重链基因(增加3倍)。这些结果表明，导电基质可以在没有额外电刺激的情况下调节成肌细胞向肌管形成的诱导，提示这些纤维可能作为骨组织工程的临时基质。(C)2009爱思唯尔有限公司。保留所有权利。
Myotubes assemble with bundles of myofibers to form the structural units in skeletal muscle. Therefore, myotube formation plays an important role in restoring muscular functions, and substrates to promote the differentiation of myoblasts to myotubes need to be developed for muscle tissue engineering. In this study, we developed electrically conductive composite fibers of poly(L-lactide-co-epsilon-caprolactone) (PLCL) blended with polyaniline (PANi) using an electrospinning method, and then investigated the effect of these composite fibers on the differentiation of myoblasts. The prepared PLCL/PANi fibers showed no significant difference in fiber diameter or contact angle, regardless of the incorporation of PANi. The fibers containing 30% PANi (PLCL/PANi-30) maintained elastic properties of maximum elongation at break (160 +/- 14.4%). The composite fibers were cytocompatible, as the DNA content on each fiber was similar for up to 8 days of C2C12 myoblast culture. After 4 days of culture, the number of cells positive for sarcomeric myosin was 3.6-times greater on the electrically conductive fibers (21 +/- 1 and 19 +/- 2 for PLCL/PANi-15 and -30 fibers, respectively) than on the PLCL/PANi-0 fibers (6 +/- 2). Furthermore, the level of myogenin expression detected on day 8 of culture on PLCL/PANi-15 was approximately 1.6-fold greater than the PLCL/PANi-0 fibers. Similar results were observed for the expression of other genes including troponin T (2-fold greater) and the myosin heavy chain gene (3-fold greater). These results indicate that electrically conductive substrates can modulate the induction of myoblasts into myotube formation without additional electrical stimulation, suggesting that these fibers may have potential as a temporary substrate for skeletal tissue engineering. (C) 2009 Elsevier Ltd. All rights reserved.