Injection of embryonic stem cells into scarred rabbit vocal folds enhances healing and improves viscoelasticity:: Short-term results
Injection of embryonic stem cells into scarred rabbit vocal folds enhances healing and improves viscoelasticity:: Short-term results
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DOI：
10.1097/mlg.0b013e3181379c7c
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发表时间：
2007-11-01
期刊：
影响因子：
2.6
通讯作者：
Hertegard, Stellan
中科院分区：
文献类型：
--
作者：
Cedervall, Jessica;Aehrlund-Richter, Lars;Hertegard, Stellan
目的：创伤造成的疤痕;癌症后的治疗或声带炎症与固有层的僵硬有关，并导致严重的声音问题。目前没有有效的治疗方法。人类胚胎干细胞（hESC）已被公认为提供细胞移植的潜在资源，但在未分化状态下，由于无意发育的风险，它们通常不被考虑用于治疗用途。本研究评估了人胚胎干细胞的功能潜力，以防止或减少瘢痕形成和改善粘弹性移植到瘢痕兔vocal folds.Study设计：人胚胎干细胞被注射到22瘢痕声带的新西兰兔。1个月后，声带解剖和分析的持久性hESC的荧光原位杂交使用人类特异性探针，并通过评估在苏木精-伊红染色组织的分化。使用平板流变仪来评价功能效果，即，结果：结果显示hESC处理的声带与未处理的声带相比，粘弹性显著改善。在hESC注射后1个月发现平均5.1%的人细胞植入。在人胚胎干细胞注射褶皱，开发兼容软骨，肌肉和上皮细胞在紧密接近或相互混合与适当的本地V兔tissue.Conclusions：组织学和位置的幸存的人胚胎干细胞衍生的细胞强烈表明，功能的改善是由注射的细胞，这是再生疤痕组织。这些发现指向宿主微环境的强烈影响，导致区域特异性体内hESC分化。三种类型组织在成年兔声带瘢痕中的再生。
Objectives: Scarring caused by trauma; postcancer treatment, or inflammation in the vocal folds is associated with stiffness of the lamina propria and results in severe voice problems. Currently there is no effective treatment. Human embryonic stem cells (hESC) have been recognized as providing a potential resource for cell transplantations, but in the undifferentiated state, they are generally not considered for therapeutic use due to risk of inadvertent development. This study assesses the functional potential of hESC to prevent or diminish scarring and improve viscoelasticity following grafting into scarred rabbit vocal folds.Study Design: hESC were injected into 22 scarred vocal folds of New Zealand rabbits. After 1 month, the vocal folds were dissected and analyzed for persistence of hESC by fluorescence in situ hybridization using a human specific probe, and for differentiation by evaluation in hematoxylin-eosin-stained tissues. Parallel-plate rheometry was used to evaluate the functional effects, i.e., viscoelastic properties, after treatment with hESC.Results: The results revealed significantly improved viscoelasticity in the hESC-treated vs. non-treated vocal folds. An average of 5.1% engraftment of human cells was found 1 month after hESC injection. In the hESC-injected folds, development compatible with cartilage, muscle and epithelia in close proximity or inter-mixed with the appropriate native V rabbit tissue was detected in combination with less scarring and improved viscoelasticity.Conclusions: The histology and location of the surviving hESC-derived cells strongly indicate that the functional improvement was caused by the injected cells, which were regenerating scarred tissue. The findings point toward a strong impact from the host microenvironment, resulting in a regional specific in vivo hESC differentiation. and regeneration of three; types of tissue in scarred vocal folds of adult rabbits.