Upregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis
Upregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis
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DOI：
10.1136/jcp.2006.039099
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发表时间：
2007-05-01
影响因子：
3.4
通讯作者：
Chan, Kwok-Wah
中科院分区：
文献类型：
--
作者：
Yuen, Hiu-Fung;Chan, Yuen-Piu;Chan, Kwok-Wah
背景：Twist的抗凋亡活性和上皮-间质转化活性分别与肿瘤转化和转移的发生有关。在几种类型的人类癌症中，Twist的上调也作为预后不良的预后标志。目的：探讨Twist在食管鳞状细胞癌（SCC）中的表达及其在中国食管鳞状细胞癌患者中的预后价值。方法：应用免疫组化染色法对87例原发性食管鳞状细胞癌中Twist的表达进行研究。western blot法检测了1株永生化正常食管上皮细胞株和6株食管鳞状细胞癌细胞株的Twist蛋白水平。采用半定量逆转录- pcr方法检测30对食管上切缘原发性食管鳞状细胞癌和非肿瘤性食管上皮冷冻标本中的Twist mRNA水平。结果：Twist在食管鳞状细胞癌细胞系中表达上调，其mRNA和蛋白水平在食管鳞状细胞癌及非肿瘤性食管上皮中均升高（p < 0.001）。此外，食管SCC中Twist的高水平表达与患者在食管切除术后1年内发生远处转移的风险显著相关（OR 3.462, 95% CI 1.201 ~ 9.978; p = 0.022）。结论：我们的研究结果表明Twist的上调在食管鳞状细胞癌的肿瘤转化和随后的远处转移发展中起作用。Twist可作为预测食管鳞状细胞癌远处转移的有效预后指标。
Background: The antiapoptotic and epithelial-mesenchymal transition activities of Twist have been implicated in the neoplastic transformation and the development of metastasis, respectively. Upregulation of Twist, described in several types of human cancer, also acts as a prognostic marker of poor outcome.Aim: To investigate Twist expression in oesophageal squamous cell carcinoma (SCC) and its prognostic value in a Chinese cohort of patients with oesophageal SCC.Methods: Twist expression in primary oesophageal SCC of 87 Chinese patients was investigated by immunohistochemical staining. Twist protein level in one immortalised normal oesophageal epithelial cell line and six oesophageal SCC cell lines was measured by western blot analysis. Twist mRNA level in 30 pairs of frozen specimens of primary oesophageal SCC and non-neoplastic oesophageal epithelium from the upper resection margin of corresponding oesophagectomy specimen was also determined by semiquantitative reverse transcription-PCR.Results: It was found that Twist was upregulated in oesophageal SCC cell lines, and its mRNA and protein levels were both increased in oesophageal SCC and the non-neoplastic oesophageal epithelium (p < 0.001). In addition, a high level of Twist expression in oesophageal SCC was significantly associated with a greater risk for the patient of developing distant metastasis within 1 year of oesophagectomy (OR 3.462, 95% CI 1.201 to 9.978; p = 0.022).Conclusions: Our results suggest that upregulation of Twist plays a role in the neoplastic transformation to oesophageal SCC and subsequent development of distant metastasis. Twist may serve as a useful prognostic marker for predicting the development of distant metastasis in oesophageal SCC.