TRANSFORMATION OF YEAST SPHEROPLASTS WITHOUT CELL-FUSION
TRANSFORMATION OF YEAST SPHEROPLASTS WITHOUT CELL-FUSION
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DOI：
10.1016/0003-2697(87)90240-5
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发表时间：
1987-06-01
影响因子：
2.9
通讯作者：
PERCIVAL, KJ
中科院分区：
文献类型：
--
作者：
BURGERS, PMJ;PERCIVAL, KJ
酿酒酵母原生质球的遗传转化效率比以前公布的方法提高了10- 100倍。单链和双链复制质粒的最佳转化频率是2 × 10 - 6。107和5 ×106个转化体/μ g。在饱和DNA浓度下，分别有12%和3%的活原生质球含有质粒DNA。已经历核融合的转化体的百分比从0.1%至3%变化，表明融合对于酵母原生质球摄取DNA是不需要的。
The efficiency of genetic transformation of Saccharyomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 .times. 107 and 5 .times. 106 transformants/.mu.g, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.