Phasor analysis for nonlinear pump-probe microscopy
Phasor analysis for nonlinear pump-probe microscopy
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DOI：
10.1364/oe.20.017082
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发表时间：
2012-07-11
期刊：
影响因子：
3.8
通讯作者：
Warren WS
中科院分区：
文献类型：
--
作者：
Robles FE;Wilson JW;Fischer MC;Warren WS
泵浦探测显微镜通过探测着色样品的瞬时、激发态动态特性来提供分子信息。暂态响应的分析通常使用主成分分析或多指数拟合，然而这些方法并不总是实用或可行的。在这里，我们展示了相量分析的改编，为分析和显示泵浦探测图像提供了一种直观、稳健和高效的方法，从而减轻了与区分多种颜料相关的一些挑战。给出了理解复杂的暂态信号如何映射到相量图的理论处理。对皮肤和眼睛色素组织样本的分析，以及ART中历史色素的分析，证明了这种方法的实用性。
Pump-probe microscopy provides molecular information by probing transient, excited state dynamic properties of pigmented samples. Analysis of the transient response is typically conducted using principal component analysis or multi-exponential fitting, however these methods are not always practical or feasible. Here, we show an adaptation of phasor analysis to provide an intuitive, robust, and efficient method for analyzing and displaying pump-probe images, thereby alleviating some of the challenges associated with differentiating multiple pigments. A theoretical treatment is given to understand how the complex transient signals map onto the phasor plot. Analyses of cutaneous and ocular pigmented tissue samples, as well as historical pigments in art demonstrate the utility of this approach.