Reduced spontaneous apoptosis in peripheral blood neutrophils during exacerbation of COPD
Reduced spontaneous apoptosis in peripheral blood neutrophils during exacerbation of COPD
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DOI：
10.1183/09031936.04.00089004
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发表时间：
2004-04-01
影响因子：
24.3
通讯作者：
Lode, H
中科院分区：
文献类型：
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作者：
Pletz, MWR;Ioanas, M;Lode, H
慢性阻塞性肺疾病（COPD）急性加重的一个主要特征是活化的中性粒细胞在支气管树中的积聚。这种现象可以通过增加的迁移和/或通过由于自发凋亡的抑制而延长的存活来解释。本研究的目的是评估急性加重期COPD患者外周血中性粒细胞的凋亡行为，包括36例急性加重期住院COPD患者和10名健康志愿者。在入院时、3-5天后和出院时采集血样。在培养18小时后，基于膜联蛋白V-PE结合和核形态学测量分离的中性粒细胞的自发性凋亡。在入院时，与健康志愿者相比，COPD患者的自发性凋亡率显著较低（平均值+/-SD 31+/-13% vs 44+/-18%）。入院时细胞凋亡的平均百分比为31+/-13%，3-5天后为39+/-15%，出院时为47+/-18%。第一天和出院时的自发性细胞凋亡率之间有统计学显著差异。一秒钟用力呼气量
A major feature of acute exacerbation of chronic obstructive pulmonary disease (COPD) is the accumulation of activated neutrophils in the bronchial tree. This phenomenon can be explained by an increased migration and/or by a prolonged survival due to an inhibition of spontaneous apoptosis. The aim of this study was to assess the apoptotic behaviour of peripheral blood neutrophils in COPD patients during an acute exacerbation.Thirty-six hospitalised COPD patients with an acute exacerbation and 10 healthy volunteers were included. Blood samples were obtained at admission, after 3-5 days and at discharge. Spontaneous apoptosis of isolated neutrophils was measured based on Annexin V-PE binding and nuclear morphology after culturing for 18 h.At admission, significantly lower rates of spontaneous apoptosis were noted in COPD patients compared with healthy volunteers (mean+/-SD 31+/-13% versus 44+/-18%). The mean percentages of apoptotic neutrophills were 31+/-13% at admission, 39+/-15% after 3-5 days and 47+/-18% at discharge. There was a statistically significant difference between the rates of spontaneous apoptosis on the first day and at discharge. Neither forced expiratory volume in one second