Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7.
Chemiluminescent aptasensor capable of rapidly quantifying Escherichia Coli O157:H7.
复制标题
DOI：
10.1016/j.talanta.2015.09.055
复制
发表时间：
2016-01
期刊：
影响因子：
6.1
通讯作者：
J. Khang;Danial Kim;K. W. Chung;Ji Hoon Lee
中科院分区：
文献类型：
--
作者：
J. Khang;Danial Kim;K. W. Chung;Ji Hoon Lee
Cost-effective and easy-to-use biosensor was developed for the rapid quantification and monitoring ofEscherichia(E.)Coli O157:H7in sample usingE. Coli O157:H7aptamer, graphene oxide (GO)/iron nanocomposites, and guanine chemiluminescence detection.E. Coli O157:H7aptamer-conjugated 6-carboxyfluorescein (6-FAM) with excellent specificity capturedE. Coli O157:H7in a sample when the mixture was incubated for 1 h at 37 °C. FreeE. Coli O157:H7 aptamers remaining in sample after the incubation were removed with GO/iron nanocomposites based on the principle of π–π stacking interaction between free aptamer and GO/iron nanocomposites. Then,E. Coli O157:H7bound with aptamer-conjugated 6-FAM in sample emitted strong light when guanine chemiluminescent reagents (e.g., 3,4,5-trimethoxylphenylglyoxal hydrate, Tetra-n-propylammonium hydroxide) were added in the sample. The strength of light emitted in guanine chemiluminescence reaction was proportionally enhanced with the increase ofE. Coli O157:H7concentration. The limit of detection (LOD) of biosensor capable of quantifyingE. Coli O157:H7with good accuracy, precision, and reproducibility was as low as 4.5×103cfu/ml. We expect that the rapid analytical system can be applied in the field of food safety as well as public health.
