Dissecting protein structure and function using directed evolution
Dissecting protein structure and function using directed evolution
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DOI：
10.1038/nmeth1207-995
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发表时间：
2007-12-01
期刊：
影响因子：
48
通讯作者：
Liu, David R.
中科院分区：
文献类型：
--
作者：
Yuen, Courtney M.;Liu, David R.
为了表征单个氨基酸对蛋白质结构或功能的贡献，研究人员采用了定向进化方法，该方法使用诱变和选择或筛选的迭代循环来搜索序列空间的广阔区域以寻找突变组，从而深入了解感兴趣的蛋白质。
To characterize the contributions of individual amino acids to the structure or function of a protein, researchers have adopted directed evolution approaches, which use iterated cycles of mutagenesis and selection or screening to search vast areas of sequence space for sets of mutations that provide insights into the protein of interest.