Solid-phase microextraction and liquid chromatography/mass spectrometry in drug analysis
Solid-phase microextraction and liquid chromatography/mass spectrometry in drug analysis
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DOI：
10.1016/s0003-2670(03)00680-9
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发表时间：
2003-09
影响因子：
6.2
通讯作者：
T. Kumazawa;X. Lee;Keizo Sato;O. Suzuki
中科院分区：
文献类型：
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作者：
T. Kumazawa;X. Lee;Keizo Sato;O. Suzuki
药物监测在很大程度上依赖于新的分析仪器或技术的发展。固相微萃取是一种新型的无溶剂样品前处理技术。SPME方法有两种不同的技术。纤维SPME是一种简单的技术，它将采样、提取、浓缩和进样集成到一个单一的程序中。纤维SPME具有许多优点，例如简单、低成本以及与气相色谱（GC）、GC/质谱（MS）、液相色谱（LC）和LC/MS的兼容性。另一种称为管内SPME的技术是SPME的自动化版本，可以很容易地连接到LC自动进样器，用于在线样品制备、分离和定量。通过这些优点，SPME现在已经在包括药物，食品，生物和药物样品在内的广泛分析领域中获得了很多兴趣。本文综述了SPME和LC/MS在生物样品（包括尿液、血液和毛发）中药物和代谢物分析中的应用。
Drug monitoring is highly dependent on the development of new analytical instruments or techniques. Solid-phase microextraction (SPME) is a new solvent-free sample preparation technique. There are two distinct techniques for the SPME method. Fiber SPME is a simple technique that integrates sampling, extraction, concentration and sample introduction into a single procedure. Fiber SPME has number of advantages such as simplicity, low cost and compatibility with gas chromatography (GC), GC/mass spectrometry (MS), liquid chromatography (LC) and LC/MS. Another technique known as in-tube SPME is an automated version of SPME that can be easily coupled to a LC autosampler for on-line sample preparation, separation and quantitation. Through these advantages, SPME has now gained a lot of interest in a broad field of analysis including drug, food, biological and pharmaceutical samples. This review is focused on the use of SPME and LC/MS for the analysis of drugs and metabolites from biological samples, including urine, blood and hair.