In vivo (1)H MRSI of glycine in brain tumors at 3T.
In vivo (1)H MRSI of glycine in brain tumors at 3T.
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DOI：
10.1002/mrm.25588
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发表时间：
2016-01
影响因子：
3.3
通讯作者：
Choi C
中科院分区：
文献类型：
--
作者：
Ganji SK;Maher EA;Choi C
人脑中甘氨酸（Gly）的磁共振波谱成像是具有挑战性的，由于丰富的相邻J-耦合共振的干扰。我们的目标是在3 T下使用优化的MR序列方案实现健康脑和脑肿瘤中Gly的可靠成像。二维1H光谱成像（SI）与点分辨光谱（PRESS）计划。160 ms的回波时间用于分离Gly和肌醇（mIns）信号。数据收集自8名健康志愿者和14名神经胶质瘤受试者。光谱分析，与LC模型，使用数值计算的基础光谱。代谢物浓度估计参考肌酸在白色物质区域在6.4 mM。从线性回归分析相对于分数灰质含量，在纯灰色和白色物质在健康的大脑中的甘氨酸浓度估计为1.1和0.3 mM，分别。Gly在肿瘤中明显升高。肿瘤与对侧Gly浓度比随着级别的升高而更广泛，显示胶质母细胞瘤中Gly升高约10倍。健康脑灰质和白色质中Gly含量有显著性差异。我们的数据表明Gly的光谱成像可以提供脑肿瘤恶性的生物标志物。
MR spectroscopic imaging of glycine (Gly) in the human brain is challenging due to the interference of the abundant neighboring J-coupled resonances. We aim to accomplish reliable imaging of Gly in healthy brain and brain tumors using an optimized MR sequence scheme at 3T. Two dimensional 1H spectroscopic imaging (SI) was performed with a point-resolved spectroscopy (PRESS) scheme. An echo time of 160 ms was used for separation between Gly and myo-inositol (mIns) signals. Data were collected from eight healthy volunteers and fourteen subjects with gliomas. Spectra were analyzed, with LCModel, using numerically-calculated basis spectra. Metabolite concentrations were estimated with reference to creatine in white matter regions at 6.4 mM. From a linear regression analysis with respect to the fractional gray matter content, the Gly concentrations in pure gray and white matter in healthy brain were estimated to be 1.1 and 0.3 mM, respectively. Gly was significantly elevated in tumors. The tumor-to-contralateral Gly concentration ratio was more extensive with higher grades, showing ~10 fold elevation of Gly in glioblastomas. The Gly level is significantly different between gray and white matter in healthy brain. Our data indicate that spectroscopic imaging of Gly may provide a biomarker of brain tumor malignancy.