Neuromodulation of intestinal transport in the suckling mouse.
Neuromodulation of intestinal transport in the suckling mouse.
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乳鼠肠道运输的神经调节。
DOI：
10.1152/ajpregu.1989.256.2.r481
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Cooke,HJ
中科院分区：
文献类型：
--
作者：
Carey,HV;Cooke,HJ
在3 ~ 4周龄乳鼠上研究了肠神经对肠道离子和糖转运的影响。将空肠的全厚度段作为平板安装在通量室中，通量室配备有电刺激肠壁中的神经。河豚毒素显着降低基础短路电流在一个子集的组织含有3-O-甲基葡萄糖在粘膜浴。内源性神经的电场刺激引起短路电流增加86 +/- 15 microA/cm 2，这是由于活性氯分泌增加，而其他离子的净运动没有显著变化。神经刺激的分泌反应被河豚毒素消除，并减少毒蕈碱拮抗剂，阿托品。胆碱能激动剂卡巴胆碱未改变3-O-甲基葡萄糖的粘膜-血清通量。这些结果表明，乳鼠空肠离子转运受肠神经系统的调节。神经刺激引起由乙酰胆碱和其他非胆碱能递质介导的氯分泌反应。小鼠小肠中的糖转运似乎不受毒蕈碱胆碱能激动剂的影响。
The influence of enteric nerves on intestinal ion and sugar transport was investigated in 3- to 4-wk-old suckling mice. Whole thickness segments of jejunum were mounted as flat sheets in flux chambers equipped to electrically stimulate nerves in the intestinal wall. Tetrodotoxin significantly reduced basal short-circuit current in a subset of tissues containing 3-O-methylglucose in the mucosal bath. Electrical field stimulation of intrinsic nerves evoked an increase in short-circuit current of 86 +/- 15 microA/cm2 that was due to an increase in active chloride secretion with no significant change in the net movement of other ions. The secretory response to neural stimulation was abolished by tetrodotoxin and reduced by the muscarinic antagonist, atropine. Mucosal-to-serosal fluxes of 3-O-methylglucose were not altered by the cholinergic agonist carbachol. These results show that ion transport in the suckling mouse jejunum is regulated by the enteric nervous system. Neural stimulation evokes a chloride secretory response that is mediated by acetylcholine and other noncholinergic transmitters. Sugar transport in the murine small intestine does not appear to be influenced by muscarinic cholinergic agonists.