Reduced Behavioral Response to Gonadal Hormones in Mice Shipped during the Peripubertal/Adolescent Period
Reduced Behavioral Response to Gonadal Hormones in Mice Shipped during the Peripubertal/Adolescent Period
复制标题
DOI：
10.1210/en.2008-1595
复制
发表时间：
2009-05-01
期刊：
影响因子：
4.8
通讯作者：
Blaustein, Jeffrey D.
中科院分区：
文献类型：
--
作者：
Laroche, Julie;Gasbarro, Lauren;Blaustein, Jeffrey D.
从商业供应商运送到实验室的动物暴露于各种各样的应激源。在青春期前后/青春期（6周龄）运送的雌性C57 B16/J小鼠，当在成年期测试时，与在成年期（12周龄）运送的雌性小鼠相比，在卵巢切除术后响应于雌二醇和孕酮注射显示出较低水平的雌性性行为。这些航运引起的女性性行为减少似乎仅限于青春期左右的脆弱时期。同样，6周龄时运送的雄性小鼠表达的雄性性行为水平低于12周龄时运送的小鼠。响应于行为测试的皮质酮水平的RIA显示，在第一次暴露于测试时，在6周龄时运送的小鼠具有降低的皮质酮水平。这些结果表明，在围青春期/青春期期间，小鼠的两种性别都容易受到与运输相关的压力源的影响。此外，他们认为，在这一时期的压力有持久的，负面的影响，对女性的雌二醇和孕酮的行为反应和男性的睾酮，它会引起下丘脑-垂体-肾上腺轴的反应的变化。这些结果表明，年龄在航运是一个关键的变量，可能会影响许多内分泌学研究，他们认为，青春期/青少年时期是一个时期的脆弱性，一些压力。（内分泌学150：2351-2358，2009）
Animals shipped from commercial suppliers to laboratories are exposed to a wide variety of stressors. Female C57Bl6/J mice shipped during the peripubertal/adolescent period (6 wk old) display lower levels of female sexual behavior in response to estradiol and progesterone injections after ovariectomy when tested in adulthood than female mice shipped in adulthood (12 wk old). These shipping-induced reductions in female sexual behavior appear to be limited to a vulnerable period around the time of puberty. Likewise, male mice shipped at 6 wk of age express lower levels of masculine sexual behavior in response to testosterone treatment as adults than do mice shipped when 12 wk old. RIA of corticosterone levels in response to behavior testing revealed that, upon first exposure to testing, mice shipped at 6 wk of age have reduced corticosterone levels. These results suggest that during the peripubertal/adolescent period, mice of both sexes are susceptible to the effects of stressors associated with shipping. Furthermore, they suggest that stress during this period has enduring, negative influences on behavioral responses to estradiol and progesterone in females and to testosterone in males, and it induces changes in response of the hypothalamic-pituitary-adrenal axis. These results suggest that age at shipping is a critical variable that may influence many endocrinological studies, and they suggest that the peripubertal/adolescent period is a period of vulnerability to some stressors. (Endocrinology 150: 2351-2358, 2009)