Generation of two human isogenic iPSC lines from fetal dermal fibroblasts.
Generation of two human isogenic iPSC lines from fetal dermal fibroblasts.
复制标题
从胎儿真皮成纤维细胞生成两个人同基因 iPSC 系
DOI：
10.1016/j.scr.2018.10.004
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发表时间：
2018
影响因子：
1.2
通讯作者：
Müller F-J
中科院分区：
文献类型：
--
作者：
Tandon R;Brändl B;Baryshnikova N;Landshammer A;Steenpass L;Keminer O;Pless O;Müller F-J
通过用附加型载体重编程胎儿真皮成纤维细胞产生两个同基因hiPSC系ZIPi 013-B和ZIPi 013-E。以前，在一项比较其他重编程方法的研究中，相同的胎儿成纤维细胞被重编程多次。因此，基因组已被多次测序。这两种新的细胞系提供了研究基础干细胞生物学和人类疾病模型的机会。它们可以作为参考细胞系，用于在充分表征的基因组背景上创建携带疾病突变的同基因克隆，因为这两种细胞系都在多个实验室中表现出出色的分化能力。资源表独特的干细胞系标识符ZIPi 013-BZIPi 013-E干细胞系的替代名称ZIP 13 K2（ZIPi013-B）ZIP13 K5（ZIPi 013-E）InstitutionZentrum für Integrative Psychiatrie gGmbH，基尔，德国经销商联系信息PD Dr. Franz-Josef Müller，franz-josef.mueller@uksh. de细胞系类型PSCOriginhuman细胞来源成纤维细胞克隆性克隆性重编程方法无转基因，附加型多系原理同基因克隆基因修饰NO修饰类型N/A相关疾病N/A基因/位点N/A修饰方法N/A转基因或抗性名称N/A诱导型/组成型系统N/A存档日期/库存日期库存日期ZIPi 013-B 2017年12月8日，库存日期ZIPi 013-E 2017年12月12日细胞系储存库/银行N/A伦理批准https：www.sciencellonline.com/technical-support/ethical-statement.htmlEthikkommission der medizinischen Fakultät der Christian-Albrechts-Universität zu基尔，批准编号A145/11
Two isogenic hiPSC lines, ZIPi013-B and ZIPi013-E, were generated by reprogramming fetal dermal fibroblasts with episomal vectors. Previously, the same fetal fibroblasts were reprogrammed multiple times in a study comparing other reprogramming methods. As a consequence, the genomes have been sequenced multiple times. Both new cell lines offer the opportunity to study basic stem cell biology and model human disease. They can be applied as reference cell lines for creating isogenic clones bearing disease mutations on a well-characterized genomic background, as both cell lines have demonstrated excellent differentiation capacity in multiple labs.Resource tableUnique stem cell lines identifierZIPi013-BZIPi013-EAlternative names of stem cell linesZIP13K2 (ZIPi013-B)ZIP13K5 (ZIPi013-E)InstitutionZentrum für Integrative Psychiatrie gGmbH, Kiel, GermanyContact information of distributorPD Dr. Franz-Josef Müller, franz-josef.mueller@uksh.deType of cell linesiPSCOriginhumanCell SourceFibroblastsClonalityClonalMethod of reprogrammingTransgene free, episomalMultiline rationaleIsogenic clonesGene modificationNOType of modificationN/AAssociated diseaseN/AGene/locusN/AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateStock date ZIPi013-B 8th December 2017, stock date ZIPi013-E 12th December 2017Cell line repository/bankN/AEthical approvalhttps://www.sciencellonline.com/technical-support/ethical-statement.htmlEthikkommission der medizinischen Fakultät der Christian-Albrechts-Universität zu Kiel, approval number A145/11