Density of platelet collagen receptors glycoprotein VI and alpha2beta1 and prior myocardial infarction in human subjects, a pilot study.
Density of platelet collagen receptors glycoprotein VI and alpha2beta1 and prior myocardial infarction in human subjects, a pilot study.
复制标题
血小板胶原蛋白受体糖蛋白 VI 和 α2β1 的密度以及人类受试者先前的心肌梗塞的初步研究。
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
M. Kahn
中科院分区：
文献类型：
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作者：
F. Samaha;C. Hibbard;Jay Sacks;Hong Chen;M. Varello;Thomas George;M. Kahn
背景
以前的研究评估血小板胶原受体是否影响心肌梗死（MI）的风险，使用了常见的多态性，认为影响受体密度，并产生了相互矛盾的结果。我们目前的初步研究的目的是获得血小板胶原受体密度和既往心肌梗死之间关系的初步信息。
材料/方法
我们测量了糖蛋白VI（GPVI）和整合素α 2 β 1密度的流式细胞术使用FITC标记的单克隆抗体在连续的受试者从门诊心脏病的做法。我们采用病例对照设计，将77例既往MI患者与77例对照患者相匹配。根据MI的传统风险因素进行匹配。
结果
既往MI患者的平均GPVI密度高14%（P = 0.098），既往MI患者的α 2 β 1密度高6%（P = 0.49）。最低十分位数内的GPVI或α 2 β 1密度与较低的MI患病率相关（OR = 6.3，95%CI：1.3-29.2 P = 0.009）。
结论
我们的初步数据表明，血小板胶原受体密度，直接测量时，可能与先前的心肌梗死。一个适当规模的临床研究，使用这种方法是必要的，以进一步研究胶原受体密度是否作为一个新的心肌梗死的危险因素。
BACKGROUND
Prior studies evaluating whether platelet collagen receptors affect risk of myocardial infarction (MI) have used common polymorphisms thought to affect receptor density, and have yielded conflicting results. Our objective in the current pilot study was to obtain preliminary information on the relationship between platelet collagen receptor density and prior MI.
MATERIAL/METHODS
We measured glycoprotein VI (GPVI) and integrin alpha2beta1 density by flow cytometry using FITC-conjugated monoclonal antibodies in consecutive subjects from an outpatient cardiology practice. We used a case-control design to match 77 patients with prior MI to 77 control patients. Matching was performed on the basis of traditional risk factors for MI.
RESULTS
The mean GPVI density was 14% higher in those with prior MI (P = 0.098), and the alpha2beta1 density was 6% higher in those with prior MI (P = 0.49). A GPVI or alpha2beta1 density within the lowest decile was associated with a lower MI prevalence (OR = 6.3, 95% CI: 1.3-29.2 P = 0.009).
CONCLUSIONS
Our preliminary data suggests that platelet collagen receptor density, when measured directly, may be related to prior myocardial infarction. An appropriately sized clinical study using this methodology is warranted to further investigate whether collagen receptor density serves as a novel risk factor for myocardial infarction.