Direct evidence for the role of pigment cells in the brain of ascidian larvae by laser ablation
Direct evidence for the role of pigment cells in the brain of ascidian larvae by laser ablation
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激光烧蚀直接证明色素细胞在海鞘幼虫大脑中的作用
DOI：
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发表时间：
2002
影响因子：
2.8
通讯作者：
M. Goda
中科院分区：
文献类型：
--
作者：
M. Tsuda;D. Sakurai;M. Goda
摘要 海鞘幼虫的前部感觉囊泡包含一个大囊泡，其中有两种不同类型的色素细胞：前部和后部。这些色素细胞的超微结构表明，前色素细胞是耳石，可能用于重力检测，后色素细胞是眼细胞，用于感光。然而，这种功能分配没有直接的实验证据。在激光消融前色素和后色素细胞之前和之后检查了幼虫生命初期发生的向上游泳行为。后部色素细胞消融的幼虫保留了向上游泳的行为，但前部色素细胞消融的幼虫失去了这种行为。这些结果表明前部色素充当重力传感器。在激光消融前色素或后色素细胞之前和之后还检查了幼虫生命后期的负趋光性游泳。前部色素细胞消融的幼虫保留了趋光反应，但后部色素细胞消融的幼虫失去了趋光反应。这些结果表明，感觉囊泡的后部色素参与负趋光性、向下游泳行为。研究了压力对游泳行为的影响，发现假定的压力检测器官与幼虫的游泳行为无关。这些是首次发表的实验结果，允许将海鞘幼虫行为的功能作用分配给感觉器官。
SUMMARY The anterior sensory vesicle of ascidian larvae contains a single large vesicle in which lie two distinct types of pigment cells, anterior and posterior. The ultrastructure of these pigment cells suggests that the anterior pigment cell is an otolith, presumably used for gravity detection, and the posterior pigment cell is an ocellus, used for photoreception. However, there is no direct experimental evidence for this assignment of function. Upward swimming behaviour occurring during the initial period of larval life was examined before and after laser ablation of the anterior pigment and posterior pigment cells. Posterior pigment cell-ablated larvae retained the upward swimming behaviour, but anterior pigment cell-ablated larvae lost it. These results suggest that the anterior pigment acts as a gravity sensor. The negatively phototactic swimming during the latter part of larval life was also examined before and after laser ablation of the anterior pigment or posterior pigment cells. Anterior pigment cell-ablated larvae retained the phototactic response, but posterior pigment cell-ablated larvae lost it. These results suggest that the posterior pigment of the sensory vesicle is involved in the negatively phototactic, downward swimming behavior. The effect of pressure on swimming behaviour was studied, and a putative pressure-detection organ was found not to be involved in the larval swimming behaviour. These are the first published experimental results that permit a functional role in ascidian larval behavior to be assigned to the sensory organs.
嗜盐杆菌趋光性中的激发信号处理时间。
影响因子：
3.4
作者：
Sundberg,SA;Alam,M;Spudich,JL
通讯作者：
Spudich,JL