Chi-miR-370-3p regulates hair follicle morphogenesis of Inner Mongolian cashmere goats.
Chi-miR-370-3p regulates hair follicle morphogenesis of Inner Mongolian cashmere goats.
复制标题
Chi-miR-370-3p调控内蒙古绒山羊毛囊形态发生
DOI：
10.1093/g3journal/jkab091
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发表时间：
2021-05-07
期刊：
影响因子：
--
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Hai E;Han W;Wu Z;Ma R;Shang F;Wang M;Liang L;Rong Y;Pan J;Wang Z;Wang R;Su R;Zhao Y;Liu Z;Wang Z;Li J;Zhang Y
摘要微小RNA（miRNAs）是一类22个核苷酸的非编码RNA，在多种生物过程中负调控mRNA转录后修饰。绒山羊皮肤毛囊的形态发生是一个涉及许多关键信号分子的动态过程，但这些关键信号分子诱导的相关细胞生物学机制尚未见报道。本研究采用差异表达、生物信息学和基因本体论/京都基因与基因组百科全书富集分析方法，对内蒙古绒山羊胎儿期45、55和65 d的miRNA表达谱进行分析，并对chi-miR-370- 3 p进行鉴定和分析。实时荧光定量（qRT-PCR）、双荧光素酶报告和Western blotting结果显示，转化生长因子β受体2（TGF-βR2）和成纤维细胞生长因子受体2（FGFR 2）是chi-miR-370- 3 p的靶基因。Chi-miR-370- 3 p还在上皮细胞和真皮成纤维细胞中在mRNA和蛋白水平上调节TGF-βR2和FGFR 2的表达。DNA染色、Cell Counting Kit-8和荧光素标记的Annexin V结果显示，chi-miR-370- 3 p抑制上皮细胞和成纤维细胞的增殖，但对凋亡没有影响。细胞划痕实验结果显示，chi-miR-370- 3 p促进了上皮细胞和成纤维细胞的迁移。Chi-miR-370- 3 p通过靶向TGF-βR2和FGFR 2抑制上皮细胞和成纤维细胞的增殖，从而提高细胞迁移能力，并最终调节上皮细胞和真皮成纤维细胞的命运以发育基板和真皮冷凝物，诱导毛囊形态发生。
Abstract MicroRNAs (miRNAs), a class of 22 nucleotide (nt) noncoding RNAs, negatively regulate mRNA posttranscriptional modification in various biological processes. Morphogenesis of skin hair follicles in cashmere goats is a dynamic process involving many key signaling molecules, but the associated cellular biological mechanisms induced by these key signaling molecules have not been reported. In this study, differential expression, bioinformatics, and Gene Ontology/Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed on miRNA expression profiles of Inner Mongolian cashmere goats at 45, 55, and 65 days during the fetal period, and chi-miR-370-3p was identified and investigated further. Real-time fluorescence quantification (qRT-PCR), dual luciferase reporting, and Western blotting results showed that transforming growth factor beta receptor 2 (TGF-βR2) and fibroblast growth factor receptor 2 (FGFR2) were the target genes of chi-miR-370-3p. Chi-miR-370-3p also regulated the expression of TGF-βR2 and FGFR2 at mRNA and protein levels in epithelial cells and dermal fibroblasts. DNA staining, Cell Counting Kit-8, and fluorescein-labelled Annexin V results showed that chi-miR-370-3p inhibited the proliferation of epithelial cells and fibroblasts but had no effect on apoptosis. Cell scratch test results showed that chi-miR-370-3p promoted the migration of epithelial cells and fibroblasts. Chi-miR-370-3p inhibits the proliferation of epithelial cells and fibroblasts by targeting TGF-βR2 and FGFR2, thereby improving cell migration ability and ultimately regulating the fate of epithelial cells and dermal fibroblasts to develop the placode and dermal condensate, inducing hair follicle morphogenesis.
影响因子：
7
作者：
Shamsaddini-Bafti M;Salehi M;Maghsoudi A;Tehrani AM;Mirzaei F;Momen SM
通讯作者：
Momen SM