Cell Viability Assays: Introduction
Cell Viability Assays: Introduction
复制标题
DOI：
10.1007/978-1-61779-108-6_1
复制
发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Stoddart, Martin J.
中科院分区：
文献类型：
--
作者：
Stoddart, Martin J.
细胞活力的测量在所有形式的细胞培养中起着重要作用。有时它是实验的主要目的，例如毒性测定。或者，细胞活力可用于将细胞行为与细胞数量相关联，从而提供例如合成代谢活性的更准确的图像。存在广泛的细胞活力方法，其范围从最常规的台盼蓝染料排除测定到个体细胞的高度复杂的分析，例如使用拉曼显微镜。所需设备的成本、速度和复杂性都将在确定所使用的测定中发挥作用。本章旨在概述目前可用的许多检测方法。
The measurement of cell viability plays a fundamental role in all forms of cell culture. Sometimes it is the main purpose of the experiment, such as in toxicity assays. Alternatively, cell viability can be used to correlate cell behaviour to cell number, providing a more accurate picture of for example, anabolic activity. There are wide arrays of cell viability methods which range from the most routine trypan blue dye exclusion assay to highly complex analysis of individual cells, such as using RAMAN microscopy. The cost, speed, and complexity of equipment required will all play a role in determining the assay used. This chapter aims to provide an overview of many of the assays available today.