Chemical mutagenesis and fine-structure functional analysis of the mouse genome.
Chemical mutagenesis and fine-structure functional analysis of the mouse genome.
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DOI：
10.1016/0168-9525(91)90016-j
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
E. Rinchik
中科院分区：
文献类型：
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作者：
E. Rinchik
目前的遗传图谱还受到定义单个基因的表型性质的限制。通常，由表型变体定义的基因座限于那些指定容易识别的特征或行为的基因座。例如，隐性致死突变，特别是那些在产前阻止发育并且在单剂量状态下不具有某种类型的（显性）效应的突变，如果不使用适当的染色体标记和特定的育种方案，很少被检测到。在果蝇的研究由一些小组已经证明，屏幕上的新的突变，包括致命的突变，通过“饱和诱变”的策略，可以帮助剖析一个特定的表型S-10的组件或整个染色体区域的功能组成11，12。因此，缩小小鼠功能分辨率差距的一种方法，至少部分地，是将高效生殖细胞诱变的方法纳入策略中，以产生，检测，维持和绘制所有类别的新突变，包括那些通常难以识别和维持的突变。fecdve细菌-cdl诱变剂
The current genetic map is also limited by the nature of phenotypes defining individual genes. Generally, loci defined by phenotypic variants are limited to those specifying easily recognizable characters or behaviors. Recessive-lethal mutations, for example, and particularly those that arrest development prenatally and do not have some type of (dominant) effect in the one-dose state, are rarely detected without the use of appropriate chromosomal markers and specific breeding schemes. Studies in Drosophila by a number of groups have demonstrated that screens for new mutations, including lethal mutations, by strategies of'saturation mutagenesis' can help to dissect the components of a particular phenotype s-10 or the functional make-up of an entire chromosomal region11, 12. Thus, one way of closing the functional resolution gap in the mouse, at least in part, is to incorporate methods of high-efficiency germ-cell mutagenesis into strategies to generate, detect, maintain and map all classes of new mutatioJ~ s, including those normally difficult to recognize and maintain.~ fecdve germ-cdl mutagens