PYROPHOSPHATASE IS ESSENTIAL FOR GROWTH OF ESCHERICHIA-COLI
PYROPHOSPHATASE IS ESSENTIAL FOR GROWTH OF ESCHERICHIA-COLI
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DOI：
10.1128/jb.172.10.5686-5689.1990
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发表时间：
1990-10-01
影响因子：
3.2
通讯作者：
PLATEAU, P
中科院分区：
文献类型：
--
作者：
CHEN, J;BREVET, A;PLATEAU, P
无机焦磷酸酶ppa基因是大肠杆菌生长所必需的。染色体ppa：：Kanr病变和ppa+基因的温度敏感复制子的重组体显示温度敏感的生长表型，并且在乳糖启动子的控制下具有唯一ppa+基因的突变体显示诱导剂依赖性生长。当lacp-ppa突变体在没有诱导剂的情况下继代培养时，焦磷酸酶水平降低，PPi水平升高，并且生长停止。细胞PPi在生长停滞后约6小时达到16 mM，而不丧失细胞活力。
The ppa gene for inorganic pyrophosphatase is essential for the growth of Escherichia coli. A recombinant with a chromosomal ppa::Kanr lesion and a temperature-sensitive replicon with a ppa+ gene showed a temperature-sensitive growth phenotype, and a mutant with the sole ppa+ gene under control of the lac promoter showed inducer-dependent growth. When the lacp-ppa mutant was subcultured without inducer, the pyrophosphatase level decreased, the PPi level increased, and growth stopped. Cellular PPi reached 16 mM about 6 h after growth arrest without loss of cell viability.