Diets Supplemented with 1% Egg White Induce Oral Desensitization and Immune Tolerance in an Egg White-Specific Allergic Mouse Model.
Diets Supplemented with 1% Egg White Induce Oral Desensitization and Immune Tolerance in an Egg White-Specific Allergic Mouse Model.
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饮食%20补充%20和%201%%20鸡蛋%20白色%20诱导%20口服%20脱敏%20和%20免疫%20耐受%20in%20an%20鸡蛋%20白色特定%20过敏%20小鼠%20模型。
DOI：
10.1159/000488858
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Takahashi K.
中科院分区：
文献类型：
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作者：
Maeta A;Matsushima M;Katahira R;Sakamoto N;Takahashi K.
本研究的目的是确定蛋清过敏小鼠模型在口服免疫治疗（OIT） 4周内诱导口腔脱敏和/或免疫耐受所需的饮食中蛋清（EW）浓度。方法对BALB/c雌性小鼠进行腹腔注射致敏，然后口服致敏原灌胃。致敏小鼠在20%酪蛋白饮食中分别添加0（非OIT）、0.01、0.1或1% EW，给予4周的OIT。非致敏小鼠作为非过敏组。我们进行了口服和腹腔EW挑战，评估了背部皮肤的血管通透性，并测量了过敏生物标志物。结果非过敏组和1% EW组口腔刺激后直肠温度变化无显著性差异，1% EW组腹泻频率低于非oit组。在研究终点，1% EW组血浆卵泡样细胞特异性IgE、IgA和IgG2a水平显著低于非oit组。1% EW组脾淋巴细胞IFN-γ和IL-10分泌量显著高于非oit组，CD4+ Foxp3+细胞百分比显著高于非oit组。结论饲粮中添加1% EW可诱导EW过敏小鼠口腔脱敏和免疫耐受。
BackgroundThe objective of this study was to determine the required concentration of egg white (EW) in the diet to induce oral desensitization and/or immune tolerance within 4 weeks of oral immunotherapy (OIT) in an EW allergic mouse model.MethodsFemale BALB/c mice were systemically sensitized to EW by intraperitoneal injections and subsequently subjected to oral allergen gavage. Sensitized mice were provided 4 weeks of OIT by supplementing with 0 (non-OIT), 0.01, 0.1, or 1% EW in a 20% casein diet. Nonsensitized mice served as the nonallergy group. We performed oral and intraperitoneal EW challenges, assessed vascular permeability in the dorsal skin, and measured allergic biomarkers.ResultsThe change in rectal temperature after oral challenge was not significantly different between the nonallergy and 1% EW groups, and the frequency of diarrhea in the 1% EW group was lower than that in the non-OIT group. The levels of plasma ovomucoid-specific IgE, IgA, and IgG2a in the 1% EW group at the study endpoint were significantly lower than those in the non-OIT group. IFN-γ and IL-10 secretions of spleen lymphocytes in the 1% EW group were significantly higher than those in the non-OIT group, and the percentage of CD4+ Foxp3+ cells in the 1% EW group was higher than that in the non-OIT group.ConclusionThese results suggested that diet supplemented with 1% EW can induce oral desensitization and immune tolerance in the EW allergic mouse model.