PCTAIRE-1 and PCTAIRE-3, two members of a novel cdc2/CDC28-related protein kinase gene family.
PCTAIRE-1 and PCTAIRE-3, two members of a novel cdc2/CDC28-related protein kinase gene family.
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发表时间：
1992-11
期刊：
影响因子：
8
通讯作者：
T. Okuda;J. Cleveland;J. Downing
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文献类型：
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作者：
T. Okuda;J. Cleveland;J. Downing
我们分离了两种小鼠 cDNA，命名为 PCTAIRE-1 和 -3，它们编码假定的丝氨酸/苏氨酸特异性蛋白激酶。 PCTAIRE-1 和 -3 的预测产物具有 65% 同源性，并被组织成一个由 295 个残基组成的核心激酶结构域，两侧分别是独特的 161 和 117 个氨基酸 N 端结构域以及 40 和 39 个氨基酸 C 端结构域。这些激酶结构域与 cdc2/CDC28 激酶基因家族的成员大约有 50-55% 同源性，并且每个激酶结构域在保守的 PSTAIRE 基序中都包含半胱氨酸对丝氨酸的取代。 PCTAIRE-1 普遍表达为主要的 3.0-kb 转录物和由差异聚腺苷酸化产生的次要的 2.2-kb mRNA。相比之下，PCTAIRE-3 表现出更受限的表达模式，在脑、肾和肠中检测到单个 3.0-kb mRNA。体外转录翻译产生的 PCTAIRE-1 和 -3 产物未能与 p13suc1 结合，但被针对粟酒裂殖酵母 p34cdc2 或人 PSTAIRE 基序的抗体沉淀。因此，PCTAIRE-1和-3是cdc2/CDC28相关蛋白激酶的新亚家族的成员。
We have isolated two murine cDNAs designated PCTAIRE-1 and -3 which encode putative serine/threonine-specific protein kinases. The predicted products of PCTAIRE-1 and -3 are 65% homologous and are organized into a core 295-residue kinase domain flanked by unique 161 and 117 amino acid N-terminal and 40 and 39 amino acid C-terminal domains respectively. The kinase domains are approximately 50-55% homologous to members of the cdc2/CDC28 kinase gene family, and each contains a cysteine-for-serine substitution within the conserved PSTAIRE motif. PCTAIRE-1 was ubiquitously expressed as a predominant 3.0-kb transcript and a minor 2.2-kb mRNA resulting from differential polyadenylation. In contrast, PCTAIRE-3 exhibited a more restricted pattern of expression with a single 3.0-kb mRNA detected in brain, kidney and intestine. The PCTAIRE-1 and -3 products produced by in vitro transcription-translation failed to bind to p13suc1 but were precipitated by antibodies directed to Schizosaccharomyces pombe p34cdc2 or to the human PSTAIRE motif. Thus, PCTAIRE-1 and -3 are members of a novel subfamily of cdc2/CDC28-related protein kinases.