REGULATION OF P-SELECTIN BY TUMOR-NECROSIS-FACTOR-ALPHA
REGULATION OF P-SELECTIN BY TUMOR-NECROSIS-FACTOR-ALPHA
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DOI：
10.1006/bbrc.1995.1643
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发表时间：
1995-05-05
影响因子：
3.1
通讯作者：
BRASEL, C
中科院分区：
文献类型：
--
作者：
BISCHOFF, J;BRASEL, C
在用或不用细胞因子肿瘤坏死因子-α处理的牛毛细血管细胞中分析P-选择素mRNA和多肽的水平。3 kb的P-选择素的mRNA上调3至5倍，在苦参碱刺激的细胞。通过代谢标记和免疫吸附测定，mRNA的增加与P-选择素多肽的急剧但短暂的增加相关。这些数据证实了小鼠内皮瘤细胞系中表达的小鼠P-选择素的早期研究，并进一步表明P-选择素的功能不仅可以通过快速易位到细胞表面来调节，还可以通过精氨酸刺激P-选择素的生物合成来调节。(C)出版社：Academic Press
The levels of P-selectin mRNA and polypeptide were analyzed in bovine capillary cells treated with or without the cytokine tumor necrosis factor-alpha. The 3 kb P-selectin mRNA was upregulated three- to five-fold in cytokine-stimulated cells. The increase in mRNA correlated with a dramatic but short-lived increase in P-selectin polypeptide as determined by metabolic-labeling and immunoadsorption. These data confirm earlier studies on mouse P-selectin expressed in a mouse endothelioma cell line and further indicate that P-selectin function can be regulated not only by rapid translocation to the cell surface but also by cytokine-stimulation of P-selectin biosynthesis. (C) 1995 Academic Press, Inc.