Molecular diagnosis of resistance to antimalarial drugs during epidemics and in war zones
Molecular diagnosis of resistance to antimalarial drugs during epidemics and in war zones
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DOI：
10.1086/422758
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发表时间：
2004-08-15
影响因子：
6.4
通讯作者：
Doumbo, OK
中科院分区：
文献类型：
--
作者：
Djimdé, AA;Dolo, A;Doumbo, OK
恶性疟原虫突变pfcrt K76 T和dhfr/dhps“五重突变体”分别是氯喹和磺胺嘧啶-乙胺嘧啶抗性的分子标记。在马里北方政治动荡地区恶性疟原虫疟疾流行期间，标准的疗效研究是不可能的，我们在横断面调查中测量了这些标志物的患病率。在80%的感染病例中，检测到pfcrt K76 T，但没有一例携带dhfr/dhps五重突变体。根据这些结果，在防治工作中用磺胺嘧啶-乙胺嘧啶取代了氯喹。这个例子说明了耐药性的分子标记如何在流行病和其他紧急情况下提供及时的数据，为疟疾控制政策提供信息。
Plasmodium falciparum mutations pfcrt K76T and the dhfr/dhps "quintuple mutant" are molecular markers of resistance to chloroquine and sulfadoxine-pyrimethamine, respectively. During an epidemic of P. falciparum malaria in an area of political unrest in northern Mali, where standard efficacy studies have been impossible, we measured the prevalence of these markers in a cross-sectional survey. In 80% of cases of infection, pfcrt K76T was detected, but none of the cases carried the dhfr/dhps quintuple mutant. On the basis of these results, chloroquine was replaced by sulfadoxine-pyrimethamine in control efforts. This example illustrates how molecular markers for drug resistance can provide timely data that inform malaria-control policy during epidemics and other emergency situations.