NEGATIVE REGULATION OF FAR1 AT THE START OF THE YEAST-CELL CYCLE
NEGATIVE REGULATION OF FAR1 AT THE START OF THE YEAST-CELL CYCLE
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DOI：
10.1101/gad.7.5.833
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发表时间：
1993-05-01
影响因子：
10.5
通讯作者：
CROSS, FR
中科院分区：
文献类型：
--
作者：
MCKINNEY, JD;CHANG, F;CROSS, FR
在萌芽酵母中，细胞周期进程和接合的相互排斥的途径之间的切换在G1期后期开始时被控制。交配信息素通过在开始之前阻止细胞处于G1期来促进接合。信息素诱导的细胞周期停滞需要一个功能性的FAR1基因。我们发现，FAR1的转录和蛋白积累在细胞周期中是独立调节的。FAR1的RNA和蛋白在G1期早期高表达，但在G1期开始急剧下降。Far1在开始时消失之前被磷酸化，这表明修饰可能针对Far1进行降解。虽然FAR1mRNA水平在S后期或G2期再次升高，但FAR1蛋白在核分裂后才能再积累到功能水平。
In budding yeast, a switch between the mutually exclusive pathways of cell cycle progression and conjugation is controlled at Start in late G1 phase. Mating pheromones promote conjugation by arresting cells in G1 phase before Start. Pheromone-induced cell cycle arrest requires a functional FAR1 gene. We have found that FAR1 transcription and protein accumulation are regulated independently during the cell cycle. FAR1 RNA and protein are highly expressed in early G1, but decline sharply at Start. Far1 is phosphorylated just before it disappears at Start, suggesting that modification may target Far1 for degradation. Although FAR1 mRNA levels rise again during late S or G2 phase, reaccumulation of Far1 protein to functional levels is restricted until after nuclear division.