Assessing Amide Proton Transfer (APT) MRI Contrast Origins in 9 L Gliosarcoma in the Rat Brain Using Proteomic Analysis.
Assessing Amide Proton Transfer (APT) MRI Contrast Origins in 9 L Gliosarcoma in the Rat Brain Using Proteomic Analysis.
复制标题
使用蛋白质组分析评估大鼠脑 9 L 胶质肉瘤的酰胺质子转移 (APT) MRI 对比起源。
DOI：
10.1007/s11307-015-0828-6
复制
发表时间：
2015-08
影响因子：
3.1
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Yan K;Fu Z;Yang C;Zhang K;Jiang S;Lee DH;Heo HY;Zhang Y;Cole RN;Van Eyk JE;Zhou J
探讨脑肿瘤中酰胺光子传递（APT）加权高强度的生化来源。7只携带9l胶质瘤的大鼠在4.7 t时成像，用冠状大鼠脑基质和组织活检打孔制备等体积的肿瘤和正常脑组织样本。从两个样品中提取总组织蛋白和细胞质亚蛋白质组。蛋白质样品采用双向凝胶电泳分析，并用质谱法鉴定丰度变化显著的蛋白质。与正常脑区相比，肿瘤中胞质蛋白浓度显著增加，但总蛋白浓度相当。肿瘤组织与正常脑组织蛋白谱差异显著。6种细胞质蛋白、4种内质网蛋白和5种分泌蛋白在肿瘤中显著上调。我们的实验证实了肿瘤中细胞质蛋白浓度的增加，并确定了几个可能导致apt加权高强度的关键蛋白。
To investigate the biochemical origin of the amide photon transfer (APT)-weighted hyperintensity in brain tumors. Seven 9 L gliosarcoma-bearing rats were imaged at 4.7 T. Tumor and normal brain tissue samples of equal volumes were prepared with a coronal rat brain matrix and a tissue biopsy punch. The total tissue protein and the cytosolic subproteome were extracted from both samples. Protein samples were analyzed using two-dimensional gel electrophoresis, and the proteins with significant abundance changes were identified by mass spectrometry. There was a significant increase in the cytosolic protein concentration in the tumor, compared to normal brain regions, but the total protein concentrations were comparable. The protein profiles of the tumor and normal brain tissue differed significantly. Six cytosolic proteins, four endoplasmic reticulum proteins, and five secreted proteins were considerably upregulated in the tumor. Our experiments confirmed an increase in the cytosolic protein concentration in tumors and identified several key proteins that may cause APT-weighted hyperintensity.