Molecularly imprinted polymer based on CdTe@SiO2 quantum dots as a fluorescent sensor for the recognition of norepinephrine.
Molecularly imprinted polymer based on CdTe@SiO2 quantum dots as a fluorescent sensor for the recognition of norepinephrine.
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DOI：
10.1039/c4an00951g
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发表时间：
2014-10
期刊：
影响因子：
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通讯作者：
Fangdi Wei;Yanzi Wu;Guanhong Xu;Yankun Gao;Jing Yang;Liping Liu;P. Zhou;Qin Hu
中科院分区：
文献类型：
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作者：
Fangdi Wei;Yanzi Wu;Guanhong Xu;Yankun Gao;Jing Yang;Liping Liu;P. Zhou;Qin Hu
以去甲肾上腺素（NE）为识别对象，构建了一种基于CdTe@SiO2量子点（QDs）的分子印迹传感器。采用傅里叶变换红外光谱、透射电子显微镜和荧光光谱等方法对CdTe@SiO2量子点表面的分子印迹聚合物（MIP）进行了表征。所合成的纳米传感器对去甲肾上腺素具有良好的选择性和亲和力。在最佳条件下，CdTe@SiO2@MIP的相对荧光强度在0.04 ~ 10 μM范围内随NE浓度的增加而线性下降。检测限为8 nM（3σ/K）。方法用于大鼠血浆中NE的测定，结果与荧光衍生法一致。方法简便、快速，可用于生物样品中去甲肾上腺素的测定。
A novel molecular imprinted sensor based on CdTe@SiO2 quantum dots (QDs) was developed for norepinephrine (NE) recognition. The molecularly imprinted polymer (MIP) on the surface of CdTe@SiO2 QDs (CdTe@SiO2@MIP) was characterized by Fourier transform infrared spectroscopy, transmission electron microscopy and fluorescence spectroscopy. The synthesized nanosensor had a distinguished selectivity and high binding affinity to NE. Under optimal conditions, the relative fluorescence intensity of CdTe@SiO2@MIP linearly decreased with increase of the concentration of NE in the range of 0.04-10 μM. The limit of detection was 8 nM (3σ/K). The proposed method was applied to the analysis of NE in rat plasma, and the result obtained by the method was in good agreement with that assayed by the fluorescence derivatization method. The method developed is simple, fast, and can be applied to the determination of NE in biological samples.