In vivo SPECT imaging of an I-131-labeled PM 2.5 mimic substitute
In vivo SPECT imaging of an I-131-labeled PM 2.5 mimic substitute
复制标题
131I 标记的 PM 2.5 微型替代品的体内 SPECT 成像
DOI：
10.1007/s41365-020-00818-2
复制
发表时间：
2020
影响因子：
2.8
通讯作者：
Chen Ming-Qing
中科院分区：
文献类型：
--
作者：
Pan Dong-Hui;Sheng Jie;Wang Xinyu;Huang Qian-Huan;Yan Jun-Jie;Wang Li-Zhen;Yang Run-Ling;Shi Dong-Jian;Xu Yu-Ping;Chen Ming-Qing
环境pm2.5对健康的影响及其潜在机制引起了相当大的兴趣。体外细胞研究和离体动物实验可能无法准确确定pm2.5颗粒的特性。为了更好地了解它们的详细机制，我们使用单光子发射断层扫描（SPECT）成像进行了体内研究。为了模拟pm2.5颗粒，用131i标记由碳酸乙烯或聚乙烯醇吡咯烷酮修饰的sio2纳米颗粒。经吸入给药后，对放射性标记颗粒在sprague dawley大鼠体内进行SPECT成像。给药后6和24 h，放射性物质在肺和气管中积累。此外，在腹部，包括肝脏和肾脏，观察到明显的放射性。体外放射自显影也证实了这一结果。本研究表明，体内SPECT成像是研究pm2.5颗粒性质的有效方法。
The health effects of ambient PM 2.5 and its potential mechanisms have generated considerable interest. In vitro cell studies and ex vivo animal experiments may not accurately determine the characteristics of PM 2.5 particles. To better understand their detailed mechanisms, we performed an in vivo study using single photon emission tomography (SPECT) imaging. To mimic the PM 2.5 particles, SiO2nanoparticles modified by ethylene carbonate or polyvinyl pyrrolidone were labeled with131I. After administration via inhalation, in vivo SPECT imaging of the radiolabeled particles in sprague dawley rats was performed. It was found that radioactivity accumulated in the lungs and trachea 6 and 24 h after administration. In addition, significant radioactivity was observed in the abdomen, including the liver and kidneys. The results were also confirmed by ex vivo autoradiography. This study revealed that in vivo SPECT imaging could be an effective method for investigating the properties of PM 2.5 particles.