Isolation and comparative study of cell-free nucleic acids from human urine
Isolation and comparative study of cell-free nucleic acids from human urine
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DOI：
10.1196/annals.1368.045
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发表时间：
2006-01-01
期刊：
影响因子：
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通讯作者：
Laktionov, Pavel P.
中科院分区：
文献类型：
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作者：
Bryzgunova, Olga E.;Skvortsova, Tatyana E.;Laktionov, Pavel P.
研究了人尿液中的无细胞核酸（NA）。健康人尿液中DNA和RNA的浓度与性别无关，分别在6 ng/mL至50 ng/mL和24 ng/mL至140 ng/mL之间。150-400 bp的DNA片段是游离DNA的主要部分，另外在男性尿液中发现的DNA片段最大可达1300 bp，在女性尿液中发现的DNA片段最大可达19 kbp。对从乳腺癌患者血液中分离的循环DNA和通过甲基化特异性PCR从其尿液中分离的无细胞DNA进行分析表明，血浆中RASSF1A和RAR β 2基因甲基化启动子的存在伴随着尿液中检测到相同的甲基化标记。获得的数据表明无细胞尿液DNA在癌症诊断中的适用性。
Cell-free nucleic acids (NA) from human urine were investigated. Concentrations of DNA and RNA in the urine of healthy people were independent of gender and were in the range of 6 ng/mL to 50 ng/mL and 24 ng/mL to 140 ng/mL, respectively. DNA fragments of 150-400 bp represent the main part of cell-free DNA, along with DNA fragments up to 1,300 bp, which were found in male urine, and DNA fragments up to 19 kbp, which were found in female urine. Analysis of circulating DNA, isolated from blood of breast cancer patients and cell-free DNA isolated from their urine by methylation-specific PCR, demonstrates that the presence of methylated promoters of RASSF1A and RAR beta 2 genes in plasma was accompanied by the detection of the same methylated markers in urine. The data obtained demonstrate applicability of cell-free urine DNA in cancer diagnostics.