Synthetic DNA for Cell‐Surface Engineering
Synthetic DNA for Cell‐Surface Engineering
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用于细胞表面工程的合成 DNA
DOI：
10.1002/ange.202010278
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发表时间：
2021
影响因子：
--
通讯作者：
Wang, Yong
中科院分区：
文献类型：
--
作者：
Shi, Peng;Wang, Yong
细胞膜不仅是一个物理屏障，而且是一个功能细胞器，调节细胞与环境之间的通信。利用合成分子或纳米结构使细胞膜功能化的能力将使细胞功能超越进化所提供的能力。本文的目的是介绍合成DNA和基于DNA的纳米结构用于细胞表面工程的最新进展。首先介绍了一价和多价表面工程的化学偶联和物理结合方法。然后，我们介绍了这些方法在许多应用中促进或抑制细胞-环境通信的应用，包括促进细胞-细胞识别，调节细胞内通路，保护治疗细胞以及感知细胞内和细胞外微环境。最后，我们总结了当前该领域存在的挑战以及解决这些挑战的潜在解决方案。
The cell membrane is not only a physical barrier, but also a functional organelle that regulates the communication between a cell and its environment. The ability to functionalize the cell membrane with synthetic molecules or nanostructures would advance cellular functions beyond what evolution has provided. The aim of this Minireview is to introduce recent progress in using synthetic DNA and DNA‐based nanostructures for cell‐surface engineering. We first introduce chemical conjugation and physical binding methods for monovalent and polyvalent surface engineering. We then introduce the application of these methods for either the promotion or inhibition of cell–environment communication in numerous applications, including the promotion of cell–cell recognition, regulation of intracellular pathways, protection of therapeutic cells, and sensing of the intracellular and extracellular microenvironments. Lastly, we summarize current challenges existing in this area and potential solutions to solve these challenges.