High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content
High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content
复制标题
DOI：
10.1099/mic.0.000772
复制
发表时间：
2019-03-01
期刊：
影响因子：
2.8
通讯作者：
Tolker-Nielsen, Tim
中科院分区：
文献类型：
--
作者：
Almblad, Henrik;Rybtke, Morten;Tolker-Nielsen, Tim
人类病原体铜绿假单胞菌可引起急性感染和慢性生物膜感染。急性毒力因子的表达受cAMP的正调控，而生物膜的形成受c-di-GMP的正调控。我们提供的证据表明，由于缺乏降解或产量增加引起的cAMP水平升高，抑制了铜绿假单胞菌生物膜的形成。camp介导的铜绿假单胞菌生物膜形成的抑制需要Vfr，并涉及c-di-GMP水平的降低，以及生物膜基质成分的减少。突变体筛选和定义敲除突变体的表征表明，c-di- gmp降解磷酸二酯酶的一个亚群参与了P. aeruginosa中camp - vrr介导的生物膜抑制。
The human pathogen Pseudomonas aeruginosa can cause both acute infections and chronic biofilm-based infections. Expression of acute virulence factors is positively regulated by cAMP, whereas biofilm formation is positively regulated by c-di-GMP. We provide evidence that increased levels of cAMP, caused by either a lack of degradation or increased production, inhibit P. aeruginosa biofilm formation. cAMP-mediated inhibition of P. aeruginosa biofilm formation required Vfr, and involved a reduction of the level of c-di-GMP, as well as reduced production of biofilm matrix components. A mutant screen and characterization of defined knockout mutants suggested that a subset of c-di-GMP-degrading phosphodiesterases is involved in cAMP-Vfr-mediated biofilm inhibition in P. aeruginosa.