Chemogenetic Modulation and Single-Photon Calcium Imaging in Anterior Cingulate Cortex Reveal a Mechanism for Effort-Based Decisions
Chemogenetic Modulation and Single-Photon Calcium Imaging in Anterior Cingulate Cortex Reveal a Mechanism for Effort-Based Decisions
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DOI：
10.1523/jneurosci.2548-19.2020
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发表时间：
2020-07-15
影响因子：
5.3
通讯作者：
Izquierdo, Alicia
中科院分区：
文献类型：
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作者：
Hart, Evan E.;Blair, Garrett J.;Izquierdo, Alicia
行政协调会牵连在努力付出和选择的基础上努力成本，但目前还不清楚它是如何调解这种成本效益评估。在这里，雄性大鼠被训练在一个渐进的比例压下任务中努力获得高价值的奖励（蔗糖颗粒）。然后在两种条件下对训练过的大鼠进行测试：无选择条件，其中挤压蔗糖是唯一可用的食物选择，以及选择条件，其中低价值奖励（实验室食物）可自由获得作为挤压蔗糖的替代品。中断ACC，通过化学发生抑制或兴奋，减少了选择，但不是在没有选择的条件下，压力。接下来，我们在选择和无选择条件下的努力行为和奖励消费阶段寻找ACC中的价值编码细胞。为此，我们使用体内小型化荧光显微镜来可靠地跟踪相同细胞的反应，并比较ACC神经元在有选择与没有选择的相同努力行为中的反应。我们发现，与无选择会话相比，在选择期间，压力和蔗糖诱发的反应显着较弱，这可能使它们更容易受到化学遗传破坏。总之，我们的干扰实验和神经记录的结果表明，ACC介导努力决策的机制是对可用选项的效用进行区分。行政协调会通过为不同选择的相对价值提供一个稳定的人口代码来管理这些选择。
The ACC is implicated in effort exertion and choices based on effort cost, but it is still unclear how it mediates this cost-benefit evaluation. Here, male rats were trained to exert effort for a high-value reward (sucrose pellets) in a progressive ratio lever-pressing task. Trained rats were then tested in two conditions: a no-choice condition where lever-pressing for sucrose was the only available food option, and a choice condition where a low-value reward (lab chow) was freely available as an alternative to pressing for sucrose. Disruption of ACC, via either chemogenetic inhibition or excitation, reduced lever-pressing in the choice, but not in the no-choice, condition. We next looked for value coding cells in ACC during effortful behavior and reward consumption phases during choice and no-choice conditions. For this, we used in vivo miniaturized fluorescence microscopy to reliably track responses of the same cells and compare how ACC neurons respond during the same effortful behavior where there was a choice versus when there was no-choice. We found that lever-press and sucrose-evoked responses were significantly weaker during choice compared with no-choice sessions, which may have rendered them more susceptible to chemogenetic disruption. Together, findings from our interference experiments and neural recordings suggest that a mechanism by which ACC mediates effortful decisions is in the discrimination of the utility of available options. ACC regulates these choices by providing a stable population code for the relative value of different options.