Factors affecting the presence of dissolved glutathione in estuarine waters.
Factors affecting the presence of dissolved glutathione in estuarine waters.
复制标题
影响河口水域溶解谷胱甘肽存在的因素。
DOI：
10.1021/es030669g
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发表时间：
2004
影响因子：
11.4
通讯作者：
D. Armstrong
中科院分区：
文献类型：
--
作者：
D. Tang;M. Shafer;D. Karner;J. Overdier;D. Armstrong
本文研究了影响河口沃茨中巯基谷胱甘肽（GSH）存在的因素。我们的研究解决了硫醇相关联，从藻类培养物的生物释放，以及铜在硫醇释放和保存中的作用。我们在美国大陆的三个不同的河口（圣地亚哥湾，恐惧角河口，和诺福克河口）的测量结果表明，溶解的GSH，目前在亚纳摩尔水平，优先分配到超滤液部分（<1 kDa）相比，溶解的有机碳（DOC）。GSH的浓度普遍增加，总铜（Cu）水平的增加，虽然大的变异性之间的河口。在30小时的曝光实验中，释放溶解的GSH从硅藻海链藻weissfloprotein到无有机配体的实验介质是一个很强的功能，添加铜浓度。在改良的Aquil培养基中，随着Cu从背景水平（0.5 nM）增加至310 nM，释放的GSH从约0.02 fmol/细胞增加至0.27 fmol/细胞。然而，当细胞在圣地亚哥湾表面沃茨水中生长时，尽管总铜浓度要高得多，但谷胱甘肽的排泄量较低（高达0.13 fmol/细胞）。在圣地亚哥湾的水原位进行的实验表明，高浓度的添加铜不稳定的GSH，而锰（II）和天然胶体促进GSH的稳定性。与此相反，在合成介质中的实验室实验表明，中等水平的添加铜增强GSH的稳定性。
We investigated factors influencing the presence of the thiol glutathione (GSH) in estuarine waters. Our study addressed thiol phase-association, the biological release from algal cultures, and the role of copper in both thiol release and preservation. Our measurements in three diverse estuaries in the continental United States (San Diego Bay, Cape Fear Estuary, and Norfolk Estuary) show that dissolved GSH, present at sub-nanomolar levels, is preferentially partitioned into the ultra-filtrate fraction (<1 kDa) in comparison with dissolved organic carbon (DOC). Concentrations of GSH generally increased with increases in total copper (Cu)levels, although large variability was observed among estuaries. In 30-h exposure experiments, release of dissolved GSH from the diatom Thalassiosira weissflogii into organic ligand-free experimental media was a strong function of added Cu concentration. The released GSH increased from about 0.02 to 0.27 fmol/cell as Cu was increased from the background level (0.5 nM) to 310 nM in the modified Aquil media. However, excretion of GSH was lower (up to 0.13 fmol/cell) when cells were grown in surface waters of San Diego Bay, despite much higher total Cu concentrations. Experiments conducted in-situ in San Diego Bay water indicated that high concentrations of added Cu destabilized GSH, while both Mn(II) and natural colloids promoted GSH stability. In contrast, laboratory experiments in synthetic media indicated that moderate levels of added Cu enhanced GSH stability.