High prevalence of pathogenic Leptospira in wild and domesticated animals in an endemic area of China
High prevalence of pathogenic Leptospira in wild and domesticated animals in an endemic area of China
复制标题
中国流行区野生和家养动物致病性钩端螺旋体患病率高
DOI：
10.1016/s1995-7645(11)60205-8
复制
发表时间：
2011-11-01
影响因子：
3.1
通讯作者：
Qin Jinhong
中科院分区：
文献类型：
--
作者：
Wang Yalin;Zeng Lingbing;Qin Jinhong
目的：了解2009年江西省野生动物和家养动物中钩端螺旋体的感染情况。研究方法：2009年10月，从中国江西省浮梁县和上饶县采集了28头水牛的尿液样品和50头猪、50条狗和38只大鼠的肾脏样品。采用聚合酶链反应（PCR）和培养分析检测钩端螺旋体。用显微镜凝集试验（MAT）对培养的分离株进行分型。结果：实验结果表明，大鼠是钩体感染的主要宿主，其次是犬。虽然16%的大鼠（6/38）是阳性的使用培养分析，PCR分析使用诊断引物G1/G2和B64 I/B64 II或lipL 32显示鉴定为阳性，分别为50%和24%的大鼠样品的钩端螺旋体DNA的存在。结论：用G1/G2引物PCR检测肾组织中钩体DNA的效率高于lipL 32引物。然而，后一种引物具有检测尿样的潜在应用。钩端螺旋体DNA在人类居住地附近的野鼠种群中惊人的高流行率强调了常规钩端螺旋体监测的实用性，最好使用PCR方法，这比传统的基于培养的方法更敏感。
Objective: To assess the prevalence of Leptospira detected in wildlife and domesticated animals in Jiangxi Province, China, in 2009. Methods: Urine samples from 28 buffaloes and kidney samples from 50 pigs, 50 dogs and 38 rats were collected from Fuliang and Shangrao Country, Jiangxi Province, China, in October 2009. Polymerase chain reaction (PCR) and culture analyses were used to detect Leptospira. The cultured isolates were typed using the microscopic agglutination test (MAT). Results: The results showed that rats potentially serve as the main reservoir of leptospiral infection, followed by dogs. Although 16% of rats (6/38) were positive using culture analysis, PCR analysis using the diagnostic primers G1/G2 and B64I/B64II or lipL32 showed identification as 50% and 24%, respectively, of the rat samples as positive for the presence of leptospiral DNA. Conclusions: PCR-based detection of leptospiral DNA in infected kidney tissues of reservoirs is more efficient when using G1/G2 primers than lipL32 primers. However, the latter primers have a potential application for detection in urine samples. The alarmingly high prevalence of leptospiral DNA in the wild rat population near human habitation underscores the utility of routine Leptospira surveillance, preferably using PCR methods, which are more sensitive than traditional culture-based methods.