Genetic Variation of Mitochondrial DNA in Chinese Populations of Pectinophora gossypiella (Lepidoptera: Gelechiidae)
Genetic Variation of Mitochondrial DNA in Chinese Populations of Pectinophora gossypiella (Lepidoptera: Gelechiidae)
复制标题
中国棉果蝇种群线粒体DNA遗传变异（鳞翅目：果蝇科）
DOI：
10.1603/en09355
复制
发表时间：
2010-08-01
影响因子：
1.7
通讯作者：
Wu, Kongming
中科院分区：
文献类型：
--
作者：
Liu, Yudi;Hou, Maolin;Wu, Kongming
棉红铃虫是一种入侵性害虫，在世界上许多棉花种植区已成功地建立种群。本研究利用COII和Nad4的线粒体DNA序列分析了中国棉蚜群体的遗传多样性和群体结构。为了比较，巴基斯坦和美国的个体也在相同的两个mtDNA区域进行了测序。极低的遗传变异，观察到在两个线粒体区域之间的所有人群进行了检查。大多数种群只含有一到两种单倍型。虽然Nad4区域表现出相对较高的单倍型多样性和核苷酸变异，范围分别为0.363至0.591和0.00078至0.00140，在这个区域中观察到的只有三个单倍型。COII和Nad4单倍型网络剃掉了一个或两个共同的单倍型，形成了一个星形的单倍型发生的中心。配对检验表明，大多数群体没有显着分化。中国人群在Nad4区域与巴基斯坦和美国人群不同。棉蚜种群遗传变异水平低是由于入侵瓶颈造成的，这可能是由于棉蚜的非迁徙生物学和农业活动的镶嵌模式所加强的。
ABSTRACT The pink bollworm Pectinophora gossypiella is an invasive pest insect that has successfully established populations in many cotton growing regions around the world. In this study, the genetic diversity and population structure of Chinese populations of P. gossypiella were evaluated using mitochondrial DNA sequence data (COII and Nad4). For comparison, individuals of Pakistan and America were also sequenced at the same two mtDNA regions. Extremely low genetic variation was observed in the two mitochondrial regions among all populations examined. Most of the populations harbored only one to two haplotypes. Although the Nad4 region showed relatively high haplotype diversity and nucleotide variation, ranging from 0.363 to 0.591 and from 0.00078 to 0.00140, respectively, there were only three haplotypes observed in this region. COII and Nad4 haplotype networks shaved one or two common haplotype (s) forming the center of a star-shaped phylogeny. Pairwise tests showed that most of the populations were not significantly differentiated from each other. The Chinese populations were differentiated from the Pakistani and American populations in the Nad4 region. The low level of population genetic variation of P. gossypiella is attributed to invasion bottlenecks, which may have been subsequently strengthened by its nonmigratory biology and the mosaic pattern of agricultural activities.