Small Stress Response Proteins in Escherichia coli: Proteins Missed by Classical Proteomic Studies
Small Stress Response Proteins in Escherichia coli: Proteins Missed by Classical Proteomic Studies
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DOI：
10.1128/jb.00872-09
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发表时间：
2010-01-01
影响因子：
3.2
通讯作者：
Storz, Gisela
中科院分区：
文献类型：
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作者：
Hemm, Matthew R.;Paul, Brian J.;Storz, Gisela
50个或更少氨基酸的蛋白质在所有生物体中的特征都很差。相应的基因是具有挑战性的可靠注释，并且难以纯化和表征小蛋白产物。由于这些技术限制，人们对小蛋白的丰度知之甚少，更不用说它们的生物学功能了。为了开始表征大肠杆菌中的这些小蛋白，我们测定了它们在各种生长条件下和暴露于应激后的积累。我们发现许多小蛋白在特定的生长条件下积累，或者是胁迫诱导的。对于一些基因，观察到的蛋白质水平的变化与已知的转录调控一致，例如编码yccB和ybgT的操纵子的ArcA激活。然而，我们还确定了新的调节，如Zur抑制ykgMO，环AMP反应蛋白（CRP）抑制azuC，和CRP激活ykgR。11个小蛋白的水平增加热休克后，至少有1个，YobF，发生在转录后水平的诱导。这些结果表明，小分子蛋白是E.并提供信息，这将是有价值的，以确定这些蛋白质的功能。
Proteins of 50 or fewer amino acids are poorly characterized in all organisms. The corresponding genes are challenging to reliably annotate, and it is difficult to purify and characterize the small protein products. Due to these technical limitations, little is known about the abundance of small proteins, not to mention their biological functions. To begin to characterize these small proteins in Escherichia coli, we assayed their accumulation under a variety of growth conditions and after exposure to stress. We found that many small proteins accumulate under specific growth conditions or are stress induced. For some genes, the observed changes in protein levels were consistent with known transcriptional regulation, such as ArcA activation of the operons encoding yccB and ybgT. However, we also identified novel regulation, such as Zur repression of ykgMO, cyclic AMP response protein (CRP) repression of azuC, and CRP activation of ykgR. The levels of 11 small proteins increase after heat shock, and induction of at least 1 of these, YobF, occurs at a posttranscriptional level. These results show that small proteins are an overlooked subset of stress response proteins in E. coli and provide information that will be valuable for determining the functions of these proteins.