DLC-1 facilitates germ granule assembly in Caenorhabditis elegans embryo.
DLC-1 facilitates germ granule assembly in Caenorhabditis elegans embryo.
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DOI：
10.1091/mbc.e21-05-0275
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发表时间：
2022-05-01
影响因子：
3.3
通讯作者：
Voronina, Ekaterina
中科院分区：
文献类型：
--
作者：
Day, Nicholas J.;Ellenbecker, Mary;Wang, Xiaobo;Voronina, Ekaterina
生殖细胞颗粒是生殖细胞发育和生育所需的RNA结合蛋白(RBPs)的细胞质集合。在秀丽线虫受精卵的前四次细胞分裂中，生殖细胞(P)颗粒的调节组装导致它们选择性地分离到未来的生殖细胞。在这里，我们研究了DLC-1在维持P颗粒完整性中的作用，DLC-1是一种中枢蛋白，参与了不同蛋白复合体的稳定和功能。我们发现DLC-1与几种核心P颗粒蛋白直接相互作用，主要发生在胚胎发育过程中。无论DLC-1是否直接与这些蛋白质相互作用，DLC-1的缺失都会破坏P颗粒成分在胚胎中组装成相分离的细胞器。最后，我们推测，在没有DLC-1的情况下，P颗粒的扩散可能独立于DLC-1的S作为动力蛋白马达的一个亚单位的功能，而不是由于细胞极性的丧失。
Germ granules are cytoplasmic assemblies of RNA-binding proteins (RBPs) required for germ cell development and fertility. During the first four cell divisions of the Caenorhabditis elegans zygote, regulated assembly of germ (P) granules leads to their selective segregation to the future germ cell. Here we investigate the role of DLC-1, a hub protein implicated in stabilization and function of diverse protein complexes, in maintaining P granule integrity. We find that DLC-1 directly interacts with several core P granule proteins, predominantly during embryogenesis. The loss of dlc-1 disrupts assembly of P granule components into phase-separated organelles in the embryos, regardless of whether or not DLC-1 directly interacts with these proteins. Finally, we infer that P granule dispersal in the absence of dlc-1 is likely independent of DLC-1’s function as a subunit of the dynein motor and does not result from a loss of cell polarity.