Noninvasive Evaluation of Injectable Chitosan/Nano-Hydroxyapatite/Collagen Scaffold via Ultrasound
Noninvasive Evaluation of Injectable Chitosan/Nano-Hydroxyapatite/Collagen Scaffold via Ultrasound
复制标题
通过超声对可注射壳聚糖/纳米羟基磷灰石/胶原支架进行无创评估
DOI：
10.1155/2012/939821
复制
发表时间：
2012-01-01
影响因子：
--
通讯作者：
Yu, Bo
中科院分区：
文献类型：
--
作者：
Chen, Yan;Li, Songjian;Yu, Bo
为了应对设计原位成型支架和具有复杂三维（3D）结构的骨再生的挑战，合成了一种基于纳米羟基磷灰石（nHA）、胶原蛋白（Col）和壳聚糖（CS）的原位成型水凝胶支架。目前，只有有限数量的技术可用于直接且无创地介导和可视化可注射生物材料的注射过程。在这项研究中，评估了超声在体内定量评估 CS/nHAC 支架中组织发育的潜力。将 CS/nHAC 支架注射到大鼠皮下组织中并评估 28 天。使用诊断技术对支架的灰度值、体积和血流量进行定量测量。本研究表明超声可用于非侵入性、非破坏性监测和评估可注射骨支架的体内特性。与CS相比，CS/nHAC支架在体内评估中表现出更大的刚度、更低的降解率和更好的血液供应。总之，超声诊断方法是评估可注射骨支架体内形成的良好工具，并有助于在骨组织工程中监测组织发育和重塑的广泛应用。
To meet the challenges of designing an in situ forming scaffold and regenerating bone with complex three-dimensional (3D) structures, an in situ forming hydrogel scaffold based on nano-hydroxyapatite (nHA), collagen (Col), and chitosan (CS) was synthesized. Currently, only a limited number of techniques are available to mediate and visualize the injection process of the injectable biomaterials directly and noninvasively. In this study, the potential of ultrasound for the quantitative in vivo evaluation of tissue development in CS/nHAC scaffold was evaluated. The CS/nHAC scaffold was injected into rat subcutaneous tissue and evaluated for 28 days. Quantitative measurements of the gray-scale value, volume, and blood flow of the scaffold were evaluated using diagnostic technique. This study demonstrates that ultrasound can be used to noninvasively and nondestructively monitor and evaluate the in vivo characteristics of injectable bone scaffold. In comparison to the CS, the CS/nHAC scaffold showed a greater stiffness, less degradation rate, and better blood supply in the in vivo evaluation. In conclusion, the diagnostic ultrasound method is a good tool to evaluate the in vivo formation of injectable bone scaffolds and facilitates the broad use to monitor tissue development and remodeling in bone tissue engineering.