Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L.
Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L.
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DOI：
10.1007/s00122-005-0154-5
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发表时间：
2006-02
影响因子：
5.4
通讯作者：
Jianwei Zhao;J. Udall;P. Quijada;C. Grau;J. Meng;T. Osborn
中科院分区：
文献类型：
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作者：
Jianwei Zhao;J. Udall;P. Quijada;C. Grau;J. Meng;T. Osborn
油菜菌核病（Sclerotinia sclerotiorum）是由油菜菌核病菌（Sclerotinia sclerotiorum）引起的油菜主要病害之一。我们报告了参与抗S的数量性状位点（QTL）的鉴定。在DH系的两个分离群体中检测到菌核病：HUA群体，来源于部分抗性的中国冬季品系（Hua dbl 2）和易感的欧洲春季品系（P1804）之间的杂交;和MS群体，来源于部分抗性的法国冬季栽培品种（Major）和易感的加拿大春季栽培品种（Stellar）。采用叶柄接种技术和枯萎天数（DW）和茎病斑长度（SLL）两种评分方法进行抗性评价。在HUA种群中检测到总共8个影响抗性的基因组区域，其中4个区域影响两种抗性措施。在MS人群中仅检测到一个影响两种测量的区域。单个QTL可解释6-22%的方差。在来自两个群体的5个QTL上，来自抗性亲本的等位基因对抗性有贡献。来自HUA群体的N2上的QTL具有最高的LOD值和R2值，并且在第一次评估中检测到SLL。在Hua dbl 2中的N12抗性等位基因在含有来自N2的含抗性部分的同源非相互转座（HNRT）的区域中检测到。这一结果表明，N12.N2 HNRT中的QTL通过增加抗性基因的剂量而增强了华dbl 2的抗性。讨论了不同遗传背景下QTL的相互关系及其与其它农艺性状的关联。
Sclerotinia stem rot, caused by fungusSclerotinia sclerotiorum, is one of the most devastating diseases in rapeseed (Brassica napusL.). We report the identification of Quantitative trait loci (QTL) involved in the resistance toS. sclerotiorumin two segregating populations of DH lines: the HUA population, derived from a cross between a partially resistant Chinese winter line (Hua dbl2) and a susceptible European spring line (P1804); and the MS population, derived from a partially resistant French winter cultivar (Major) and a susceptible Canadian spring cultivar (Stellar). A petiole inoculation technique and two scoring methods, days to wilt (DW) and stem lesion length (SLL), were used for the resistance assessment. A total of eight genomic regions affecting resistance were detected in the HUA population, with four of these regions affecting both measures of resistance. Only one region, which affected both measurements, was detected in the MS population. Individual QTL explained 6–22% of the variance. At five of the QTL from both populations, alleles from the resistant parent contributed to the resistance. QTL on N2 from the HUA population had the highest LOD score andR2value and was detected for SLL in the first evaluation. The N12 resistance allele in Hua dbl2 was detected in a region containing a homeologous non-reciprocal transposition (HNRT) from the resistance-containing portion of N2. This result suggests that QTL in the N12.N2 HNRT enhanced the resistance of Hua dbl2 by increasing the dosage of resistance genes. The relationship of QTL from different genetic backgrounds and their associations with other agronomic traits are discussed.