Identification, Characterization, and Regulatory Mechanisms of a Novel EGR1 Splicing Isoform
Identification, Characterization, and Regulatory Mechanisms of a Novel EGR1 Splicing Isoform
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DOI：
10.3390/ijms20071548
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发表时间：
2019-03-28
影响因子：
5.6
通讯作者：
Donizetti, Aldo
中科院分区：
文献类型：
--
作者：
Aliperti, Vincenza;Sgueglia, Giulia;Donizetti, Aldo
EGR 1是在许多细胞类型中表达的转录因子，其调节参与不同生物过程（包括生长、增殖和凋亡）的基因。EGR 1表达的失调与许多病理状况如肿瘤和脑部疾病有关。控制EGR 1功能的已知分子机制包括转录、mRNA和蛋白质稳定性的调节以及翻译后修饰。在这里，我们描述了人类EGR 1基因的剪接异构体的鉴定。新鉴定的剪接转录本编码一个较短的蛋白质相比，典型的EGR 1。这种同种型缺乏属于N-末端激活结构域的区域，尽管它能够进入细胞核，但相对于典型同种型，它不能完全激活转录。
EGR1 is a transcription factor expressed in many cell types that regulates genes involved in different biological processes including growth, proliferation, and apoptosis. Dysregulation of EGR1 expression has been associated with many pathological conditions such as tumors and brain diseases. Known molecular mechanisms underlying the control of EGR1 function include regulation of transcription, mRNA and protein stability, and post-translational modifications. Here we describe the identification of a splicing isoform for the human EGR1 gene. The newly identified splicing transcript encodes a shorter protein compared to the canonical EGR1. This isoform lacks a region belonging to the N-terminal activation domain and although it is capable of entering the nucleus, it is unable to activate transcription fully relative to the canonical isoform.