Cysteine, histidine and glycine exhibit anti-inflammatory effects in human coronary arterial endothelial cells
Cysteine, histidine and glycine exhibit anti-inflammatory effects in human coronary arterial endothelial cells
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DOI：
10.1111/j.1365-2249.2011.04519.x
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发表时间：
2012-02-01
影响因子：
4.6
通讯作者：
Furukawa, S.
中科院分区：
文献类型：
--
作者：
Hasegawa, S.;Ichiyama, T.;Furukawa, S.
核因子-κ B（NF-？B）可能参与血管炎或动脉粥样硬化等血管病变的发生。最近，据报道，一些氨基酸表现出抗炎作用。我们研究了一组氨基酸对细胞因子产生或粘附分子表达的抑制作用，这些细胞因子或粘附分子参与各种细胞类型的炎症性疾病。NF-？B测定人冠状动脉内皮细胞（HCAECs），因为NF-？B调节许多细胞因子的产生和粘附分子的表达。我们研究了抑制作用的氨基酸半胱氨酸，组氨酸和甘氨酸的诱导NF-？用肿瘤坏死因子（TNF）-α刺激HCAECs，观察B活化、CD 62 E（E-选择素）表达和白细胞介素（IL）-6产生。半胱氨酸，组氨酸和甘氨酸显着减少NF-？B激活和抑制剂？Ba（I？Ba）降解。此外，所有氨基酸均能抑制HCAECs E-选择素的表达和IL-6的产生，其中半胱氨酸的作用最为显著。我们的研究结果表明，甘氨酸，组氨酸和半胱氨酸可以抑制NF-？B激活，I？Ba降解，CD 62 E表达和IL-6的产生，表明这些氨基酸可能在内皮炎症过程中表现出抗炎作用。
The activation of nuclear factor-kappa B (NF-?B) in vascular endothelial cells may be involved in vascular pathogeneses such as vasculitis or atherosclerosis. Recently, it has been reported that some amino acids exhibit anti-inflammatory effects. We investigated the inhibitory effects of a panel of amino acids on cytokine production or expression of adhesion molecules that are involved in inflammatory diseases in various cell types. The activation of NF-?B was determined in human coronary arterial endothelial cells (HCAECs) because NF-?B modulates the production of many cytokines and the expression of adhesion molecules. We examined the inhibitory effects of the amino acids cysteine, histidine and glycine on the induction of NF-?B activation, expression of CD62E (E-selectin) and the production of interleukin (IL)-6 in HCAECs stimulated with tumour necrosis factor (TNF)-a. Cysteine, histidine and glycine significantly reduced NF-?B activation and inhibitor ?Ba (I?Ba) degradation in HCAECs stimulated with TNF-a. Additionally, all the amino acids inhibited the expression of E-selectin and the production of IL-6 in HCAECs, and the effects of cysteine were the most significant. Our results show that glycine, histidine and cysteine can inhibit NF-?B activation, I?Ba degradation, CD62E expression and IL-6 production in HCAECs, suggesting that these amino acids may exhibit anti-inflammatory effects during endothelial inflammation.