STIMULATION OF PHOSPHOLIPID METHYLATION, CA2+ INFLUX, AND HISTAMINE-RELEASE BY BRIDGING OF IGE RECEPTORS ON RAT MAST-CELLS
STIMULATION OF PHOSPHOLIPID METHYLATION, CA2+ INFLUX, AND HISTAMINE-RELEASE BY BRIDGING OF IGE RECEPTORS ON RAT MAST-CELLS
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DOI：
10.1073/pnas.77.4.1903
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
AXELROD, J
中科院分区：
文献类型：
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作者：
ISHIZAKA, T;HIRATA, F;AXELROD, J
用抗IG[免疫球蛋白]E受体抗体（抗RBL [大鼠嗜碱性白血病细胞]）或抗IgE刺激正常大鼠肥大细胞;检测[3 H]甲基掺入磷脂、45 Ca摄取和组胺释放。抗RBL或其F（ab“）2片段和抗IgE诱导[3 H]甲基掺入磷脂、45 Ca内流和组胺释放增加。抗RBL的Fab“单体片段不诱导这些反应。脂质中[3 H]甲基掺入的瞬时增加在加入抗RBL或抗IgE后15 s内达到峰值，并在30 s内降至基础水平。其次是流入的45钙，增加到最大值在2分钟和组胺释放，达到最大值在3分钟。抑制磷脂甲基化导致抑制45钙流入和组胺释放。大鼠肥大细胞中的磷脂甲基化显然是由细胞表面上的IgE受体的桥接诱导的，并且磷脂甲基化的增加为Ca 2+的流入和随后的组胺的释放设置了阶段。
Normal rat mast cells were stimulated by antibodies against Ig[immunoglobulin]E receptors (anti-RBL [rat basophilic leukemia cells]) or by anti-IgE; [3H]methyl group incorporation into phospholipids, 45Ca uptake and histamine release were examined. Anti-RBL or its F(ab'')2 fragments and anti-IgE induced an increase in the incorporation of [3H]methyl into phospholipids, in 45Ca influx and in histamine release. Fab'' monomer fragments of anti-RBL induced none of these reactions. The transient increase of [3H]methyl incorporation in lipids peaked within 15 s after the addition of anti-RBL or anti-IgE and fell to basal level in 30 s. This was followed by an influx of 45Ca that increased to a maximum in 2 min and by histamine release that reached a maximum in 3 min. Inhibition of phospholipid methylation resulted in an inhibition of 45Ca influx and histamine release. Phospholipid methylation in rat mast cells is apparently induced by bridging of IgE receptors on the cell surface and that increased methylation of phospholipids sets the stage for an influx of Ca2+ and subsequent release of histamine.