Detection and identification of bacterial cell surface proteins by fluorescent labeling.
Detection and identification of bacterial cell surface proteins by fluorescent labeling.
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荧光标记检测和鉴定细菌细胞表面蛋白。
DOI：
10.1002/pmic.200600175
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发表时间：
2007
期刊：
影响因子：
3.4
通讯作者：
Lewis,JaninaP
中科院分区：
文献类型：
--
作者：
Anaya,Cecilia;Church,Nadia;Lewis,JaninaP
为了更好地了解细菌与宿主成分的相互作用，需要一种快速检测和鉴定细菌细胞表面蛋白的方法。为了检测细胞表面蛋白，我们用荧光花青染料 Cy3 和 Cy5 标记了革兰氏阴性厌氧细菌牙龈卟啉单胞菌的细胞。我们证明只有细胞表面蛋白被标记，这表明我们研究中应用的方法适用于检测和鉴定革兰氏阴性细菌和可能的其他生物体中的细胞表面蛋白。
A rapid method of detection and identification of bacterial cell surface proteins is needed to better understand the interaction of bacteria with host components. To detect cell surface proteins, we have labeled cells of the Gram‐negative anaerobic bacterium,Porphyromonas gingivalis, with fluorescent cyanine dyes, Cy3 and Cy5. We demonstrate that only cell surface proteins were labeled, indicating the method applied in our study is suitable for detection and identification of cell surface proteins in Gram‐negative bacteria and possibly other organisms.