Advantageous uses of mass spectrometry for the quantification of proteins.
Advantageous uses of mass spectrometry for the quantification of proteins.
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DOI：
10.1155/2013/219452
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发表时间：
2013
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影响因子：
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通讯作者：
Hale JE
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文献类型：
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作者：
Hale JE
通过质谱法定量蛋白质测量在研究环境中获得了广泛的接受。然而，质谱蛋白质测定的临床应用并没有随之而来。这部分是由于管理机构长期以来接受免疫学测定，如ELISA测定。在大多数情况下，ELISA为许多分析物提供高度准确、灵敏、相对便宜和简单的测定。在这些情况下接受质谱法的障碍仍然很高。然而，质谱法为某些蛋白质测量提供了解决方案，这些蛋白质测量很难（如果不是不可能的话）通过免疫学方法完成。质谱法可以为困难的测定开发提供解决方案的情况包括区分非常密切相关的蛋白质种类，以及监测由于样品处理和非常高的多路复用能力而导致的生物和分析变异性。本文将重点介绍几个例子，其中质谱法使某些蛋白质测量成为可能，免疫技术有很大的困难。
Quantitative protein measurements by mass spectrometry have gained wide acceptance in research settings. However, clinical uptake of mass spectrometric protein assays has not followed suit. In part, this is due to the long-standing acceptance by regulatory agencies of immunological assays such as ELISA assays. In most cases, ELISAs provide highly accurate, sensitive, relatively inexpensive, and simple assays for many analytes. The barrier to acceptance of mass spectrometry in these situations will remain high. However, mass spectrometry provides solutions to certain protein measurements that are difficult, if not impossible, to accomplish by immunological methods. Cases where mass spectrometry can provide solutions to difficult assay development include distinguishing between very closely related protein species and monitoring biological and analytical variability due to sample handling and very high multiplexing capacity. This paper will highlight several examples where mass spectrometry has made certain protein measurements possible where immunological techniques have had a great difficulty.